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The Mechanism of Interleukin 33-Induced Stimulation of Interleukin 6 in MLO-Y4 Cells

Noguchi, Sae ; Yamasaki, Ryota ; Nagai-Yoshioka, Yoshie ; Sato, Tsuyoshi ; Kuroishi, Kayoko ; Gunjigake, Kaori ; Ariyoshi, Wataru ; Kawamoto, Tatsuo

International journal of molecular sciences, 2023-10, Vol.24 (19), p.14842 [Periódico revisado por pares]

Basel: MDPI AG

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  • Título:
    The Mechanism of Interleukin 33-Induced Stimulation of Interleukin 6 in MLO-Y4 Cells
  • Autor: Noguchi, Sae ; Yamasaki, Ryota ; Nagai-Yoshioka, Yoshie ; Sato, Tsuyoshi ; Kuroishi, Kayoko ; Gunjigake, Kaori ; Ariyoshi, Wataru ; Kawamoto, Tatsuo
  • Assuntos: Antigens ; bone remodeling ; Chromatin ; Cytokines ; Genes ; IL-33 ; IL-6 ; Interleukins ; Kinases ; MAPK ; Metabolism ; NF-κB ; Orthodontics ; osteocyte ; Periodontium ; Phosphorylation ; Proteins
  • É parte de: International journal of molecular sciences, 2023-10, Vol.24 (19), p.14842
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The differentiation and function of osteocytes are controlled by surrounding cells and mechanical stress; however, the detailed mechanisms are unknown. Recent findings suggest that IL-33 is highly expressed in periodontal tissues in orthodontic tooth movement. The present study aimed to elucidate the effect of IL-33 on the expression of regulatory factors for bone remodeling and their molecular mechanisms in the osteocyte-like cell line MLO-Y4. MLO-Y4 cells were treated with IL-33, and the activation of intracellular signaling molecules and transcriptional factors was determined using Western blot analysis and chromatin immunoprecipitation assay. IL-33 treatment enhanced the expression of IL-6 in MLO-Y4 cells, which was suppressed by the knockdown of the IL-33 receptor ST2L. Additionally, IL-33 treatment induced activation of NF-κB, JNK/AP-1, and p38 MAPK signaling pathways in MLO-Y4 cells. Moreover, pretreatment with specific inhibitors of NF-κB, p38 MAPK, and JNK/AP-1 attenuated the IL-33-induced expression of IL-6. Furthermore, chromatin immunoprecipitation indicated that IL-33 increased c-Jun recruitment to the IL-6 promoter. Overall, these results suggest that IL-33 induces IL-6 expression and regulates osteocyte function via activation of the NF-κB, JNK/AP-1, and p38 MAPK pathways through interaction with ST2L receptors on the plasma membrane.
  • Editor: Basel: MDPI AG
  • Idioma: Inglês
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