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Gene transfer into muscle by electroporation in vivo

Aihara, Hiroyuki ; Miyazaki, Jun-ichi

Nature biotechnology, 1998-09, Vol.16 (9), p.867-870 [Peer Reviewed Journal]

New York, NY: Nature

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Title:
Gene transfer into muscle by electroporation in vivo
Author: Aihara, Hiroyuki ; Miyazaki, Jun-ichi
Subjects: Animals ; Biological and medical sciences ; Biotechnology ; Bupivacaine - pharmacology ; DNA - administration & dosage ; Electroporation ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene transfer ; Gene Transfer Techniques ; Genetic engineering ; Genetic technics ; Injections, Intramuscular ; Interleukin-5 - genetics ; Lac Operon ; Methods. Procedures. Technologies ; Mice ; Mice, Inbred C57BL ; Muscles - metabolism ; Plasmids ; Synthetic digonucleotides and genes. Sequencing
Is Part Of: Nature biotechnology, 1998-09, Vol.16 (9), p.867-870
Notes: ObjectType-Article-2
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Description: Among the nonviral techniques for gene transfer in vivo, the direct injection of plasmid DNA into muscle is simple, inexpensive, and safe. Applications of this method have been limited by the relatively low expression levels of the transferred gene. We investigated the applicability of in vivo electroporation for gene transfer into muscle, using plasmid DNA expressing interleukin-5 (IL-5) as the vector. The tibialis anterior muscles of mice were injected with the plasmid DNA, and then a pair of electrode needles were inserted into the DNA injection site to deliver electric pulses. Five days later, the serum IL-5 levels were assayed. Mice that did not receive electroporation had serum levels of 0.2 ng/ml. Electroporation enhanced the levels to over 20 ng/ml. Histochemical analysis of muscles injected with a lacZ expression plasmid showed that in vivo electroporation increased both the number of muscle fibers taking up plasmid DNA and the copy number of plasmids introduced into the cells. These results demonstrate that gene transfer into muscle by electroporation in vivo is more efficient than simple intramuscular DNA injection.
Publisher: New York, NY: Nature
Language: English

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Gene transfer into muscle by electroporation in vivo

Aihara, Hiroyuki ; Miyazaki, Jun-ichi

New York, NY: Nature

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