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Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria?

S M Muxel A P F Rosário; L R Sardinha; José Maria Alvarez; Maria Regina D'Império Lima; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)

Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006

São Paulo 2006

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  • Título:
    Which is the feature of NK1.'1 POT.+' T cells responding to acute Plasmodium chabaudi malaria?
  • Autor: S M Muxel
  • A P F Rosário; L R Sardinha; José Maria Alvarez; Maria Regina D'Império Lima; Meeting of the Brazilian Society for Immunology (31. 2006 Búzios)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts São Paulo, SP: Brazilian Society for Immunology, 2006
  • Notas: Disponível em CD-ROM
  • Descrição: Introduction and Objectives : Plasmodium infection results in activation of T, NKT and NK lymphocytes, which has been implied in the initial control of parasitemia, through IFN- ã - mediated effector mechanisms. NKT cells can be distinguished by the expression of NK cell markers such as CD161 (NK1.1), a molecule involved in cell activation, IFN-ã production and cytotoxicity. However, NKT cells represent a heterogeneous population since not all CD1drestricted T cells express NK1.1 and some NK1.1+ T cells are not restricted by CD1d. Moreover, conventional T cells increase NK1.1 expression when activated, whereas a decrease is observed in CD1d-restricted T cells. Thus, this study aims to characterize NK1.1+ T cells in the acute response to P. chabaudi. Methods and Results : M ultiparametric flow cytometric analysis of splenocytes obtained from C57BL/6 mice on days 0, 4 and 7 of infection with 106 P. chabaudi parasitized erythrocytes revealed an increase of CD3+NK1.1+ cell frequency at the different time points, being the total number of these cells higher on day 7. Among CD3+NK1.1+ cells, CD4+, CD8+ and CD4-CD8- cells represented 35,83%, 21,38% and 42,79%, respectively. Numbers similar to those were found in infected CD1dKO mice. CD4+NK1.1+ and CD8+NK1.1+ cells showed an activated phenotype (CD69 high, CD45RBhigh, CD44high, and CD62Lhigh) and produced IFN-ã. A higher expression of Fas and FasL was detected in CD8+NK1.1+ cells at day 7 of
    infection. Analysis of CFSE-labeled cells kept in culture for 72h showed lower spontaneous proliferation of NK1.1+ cells compared to NK1.1- cells, for both CD4+ and CD8+ lymphocytes. When stimulated with plate-bound anti-CD3 antibodies, a strong proliferative response occurred at day 0, decreasing after infection with NK1.1+ cells showing the lowest scores. Conclusion: These results suggest that CD4+NK1.1+ and CD8+NK1.1+ cells have effector functions that may contribute to the acute immune response to P. chabaudi. Keywords : Plasmodium chabaudi, T lymphocytes, NK1.1 molecule.
  • Editor: São Paulo
  • Data de criação/publicação: 2006
  • Formato: res. ID.055.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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