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Collagen Deposition in a Non-Fibrotic Lung Granuloma Model after Nitric Oxide Inhibition

Hogaboam, Cory M. ; Gallinat, Chad S. ; Bone-Larson, Cynthia ; Chensue, Stephen W. ; Lukacs, Nicholas W. ; Strieter, Robert M. ; Kunkel, Steven L.

The American journal of pathology, 1998-12, Vol.153 (6), p.1861-1872 [Periódico revisado por pares]

Bethesda, MD: Elsevier Inc

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Título:
Collagen Deposition in a Non-Fibrotic Lung Granuloma Model after Nitric Oxide Inhibition
Autor: Hogaboam, Cory M. ; Gallinat, Chad S. ; Bone-Larson, Cynthia ; Chensue, Stephen W. ; Lukacs, Nicholas W. ; Strieter, Robert M. ; Kunkel, Steven L.
Assuntos: Animals ; Biological and medical sciences ; Cells, Cultured ; Chemokine CCL11 ; Chemokine CCL2 - metabolism ; Chemokine CCL3 ; Chemokine CCL4 ; Chemokines, CC ; Collagen - metabolism ; Cytokines - metabolism ; Enzyme Inhibitors - pharmacology ; Female ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Granuloma, Respiratory Tract - chemically induced ; Granuloma, Respiratory Tract - metabolism ; Granuloma, Respiratory Tract - pathology ; Guanidines - pharmacology ; Interferon-gamma - metabolism ; Interleukins - metabolism ; Lung Diseases - chemically induced ; Lung Diseases - metabolism ; Lung Diseases - pathology ; Macrophage Inflammatory Proteins - metabolism ; Medical sciences ; Mice ; Mice, Inbred CBA ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - physiology ; Pneumology ; Procollagen - genetics ; Procollagen - metabolism ; Receptors, CCR2 ; Receptors, CCR3 ; Receptors, Chemokine - metabolism ; Regular ; Respiratory system : syndromes and miscellaneous diseases ; Specific Pathogen-Free Organisms ; Transforming Growth Factor beta - metabolism ; Tuberculin
É parte de: The American journal of pathology, 1998-12, Vol.153 (6), p.1861-1872
Descrição: Recent studies support the concept that pulmonary granulomatous inflammation directed by interferon (IFN)-γ, interleukin (IL)-12, and nitric oxide usually resolves in the absence of fibrosis. To determine whether nitric oxide participates in modulating the fibrotic response during the development of pulmonary granulomas in response to purified protein derivative (PPD), mice presensitized to PPD received daily intraperitoneal injections of N G-nitro- d-arginine-methyl ester ( d-NAME), N G-nitro- l-arginine-methyl ester ( l-NAME), or aminoguanidine after delivery of PPD-coated beads to the lungs. Eight days later, morphometric analysis of lung granulomas revealed that l-NAME-treated mice when challenged with PPD in vitro for 36 hours had the largest pulmonary granulomas and the greatest collagen deposition among the treated groups. In addition, equivalent numbers of dispersed lung cells from l-NAME- and aminoguanidine-treated mice produced significantly higher levels of IL-4, monocyte chemoattractant protein (MCP)-1, and macrophage inflammatory protein (MIP)-1α and significantly lower levels of eotaxin compared with d-NAME-treated mice. Cultures of dispersed lung cells from l-NAME-treated mice also produced significantly more IL-10 and less IL-12 compared with similar numbers of dispersed lung cells from d-NAME-treated mice. Cultures of isolated lung fibroblasts from l-NAME-treated mice expressed higher levels of C-C chemokine receptor 2 (CCR2) and CCR3 mRNA and contained less MCP-1 and eotaxin protein than a similar number of fibroblasts from d-NAME-treated mice. Thus, nitric oxide appears to regulate the deposition of extracellular matrix in lung granulomas through the modulation of the cytokine and chemokine profile of these lesions. Alterations in the cytokine, chemokine, and procollagen profile of this lesion may be a direct effect of nitric oxide on the pulmonary fibroblast and provide an important signal for regulating fibroblast activity during the evolution of chronic lung disease.
Editor: Bethesda, MD: Elsevier Inc
Idioma: Inglês

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Collagen Deposition in a Non-Fibrotic Lung Granuloma Model after Nitric Oxide Inhibition

Hogaboam, Cory M. ; Gallinat, Chad S. ; Bone-Larson, Cynthia ; Chensue, Stephen W. ; Lukacs, Nicholas W. ; Strieter, Robert M. ; Kunkel, Steven L.

Bethesda, MD: Elsevier Inc

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