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Vaccination of Gerbils with Bm-103 and Bm-RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection

Arumugam, Sridhar ; Wei, Junfei ; Liu, Zhuyun ; Abraham, David ; Bell, Aaron ; Bottazzi, Maria Elena ; Hotez, Peter J ; Zhan, Bin ; Lustigman, Sara ; Klei, Thomas R Mitre, Edward

PLoS neglected tropical diseases, 2016-04, Vol.10 (4), p.e0004586-e0004586 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Vaccination of Gerbils with Bm-103 and Bm-RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection
Autor: Arumugam, Sridhar ; Wei, Junfei ; Liu, Zhuyun ; Abraham, David ; Bell, Aaron ; Bottazzi, Maria Elena ; Hotez, Peter J ; Zhan, Bin ; Lustigman, Sara ; Klei, Thomas R
Mitre, Edward
Assuntos: Adjuvants, Immunologic - administration & dosage ; Alum Compounds - administration & dosage ; Animals ; Antibodies, Helminth - blood ; Antigens ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Biology and Life Sciences ; Brugia malayi - genetics ; Brugia malayi - immunology ; Causes of ; Disease ; Disease Models, Animal ; Dosage and administration ; Experiments ; Filariasis - prevention & control ; Fusion proteins ; Gerbillinae ; Health aspects ; Immunization ; Infections ; Medicine and Health Sciences ; Nematoda ; Parasite Load ; Parasite vaccines ; People and Places ; Physiological aspects ; Proteins ; Recombinant Proteins - administration & dosage ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Roundworms ; Standard deviation ; Studies ; Treatment Outcome ; Tropical diseases ; Vaccines ; Vaccines, Subunit - administration & dosage ; Vaccines, Subunit - genetics ; Vaccines, Subunit - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - genetics ; Vaccines, Synthetic - immunology ; Worms
É parte de: PLoS neglected tropical diseases, 2016-04, Vol.10 (4), p.e0004586-e0004586
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: TRK SL DA MEB PJH SA. Performed the experiments: SA BZ JW ZL AB. Analyzed the data: SA. Contributed reagents/materials/analysis tools: SA BZ JW ZL SL. Wrote the paper: SA.
The authors have declared that no competing interests exist.
Descrição: The Brugia malayi Bm-103 and Bm-RAL-2 proteins are orthologous to Onchocerca volvulus Ov-103 and Ov-RAL-2, and which were selected as the best candidates for the development of an O. volvulus vaccine. The B. malayi gerbil model was used to confirm the efficacy of these Ov vaccine candidates on adult worms and to determine whether their combination is more efficacious. Vaccine efficacy of recombinant Bm-103 and Bm-RAL-2 administered individually, concurrently or as a fusion protein were tested in gerbils using alum as adjuvant. Vaccination with Bm-103 resulted in worm reductions of 39%, 34% and 22% on 42, 120 and 150 days post infection (dpi), respectively, and vaccination with Bm-RAL-2 resulted in worm reductions of 42%, 22% and 46% on 42, 120 and 150 dpi, respectively. Vaccination with a fusion protein comprised of Bm-103 and Bm-RAL-2 resulted in improved efficacy with significant reduction of worm burden of 51% and 49% at 90 dpi, as did the concurrent vaccination with Bm-103 and Bm-RAL-2, with worm reduction of 61% and 56% at 90 dpi. Vaccination with Bm-103 and Bm-RAL-2 as a fusion protein or concurrently not only induced a significant worm reduction of 61% and 42%, respectively, at 150 dpi, but also significantly reduced the fecundity of female worms as determined by embryograms. Elevated levels of antigen-specific IgG were observed in all vaccinated gerbils. Serum from gerbils vaccinated with Bm-103 and Bm-RAL-2 individually, concurrently or as a fusion protein killed third stage larvae in vitro when combined with peritoneal exudate cells. Although vaccination with Bm-103 and Bm-RAL-2 individually conferred protection against B. malayi infection in gerbils, a more consistent and enhanced protection was induced by vaccination with Bm-103 and Bm-RAL-2 fusion protein and when they were used concurrently. Further characterization and optimization of these filarial vaccines are warranted.
Editor: United States: Public Library of Science
Idioma: Inglês

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Vaccination of Gerbils with Bm-103 and Bm-RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection

Arumugam, Sridhar ; Wei, Junfei ; Liu, Zhuyun ; Abraham, David ; Bell, Aaron ; Bottazzi, Maria Elena ; Hotez, Peter J ; Zhan, Bin ; Lustigman, Sara ; Klei, Thomas R Mitre, Edward

United States: Public Library of Science

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