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Construction of cloning vectors for Bacillus thuringiensis

Arantes, Olivia ; Lereclus, Didier

Gene, 1991-12, Vol.108 (1), p.115-119 [Periódico revisado por pares]

Lausanne: Elsevier B.V

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Título:
Construction of cloning vectors for Bacillus thuringiensis
Autor: Arantes, Olivia ; Lereclus, Didier
Assuntos: Bacillus subtilis ; Bacillus thuringiensis ; Bacillus thuringiensis - genetics ; bacterial insecticides ; Bacterial Proteins ; Bacterial Toxins ; Biological and medical sciences ; Biotechnology ; cloning ; Cloning, Molecular ; delta-endotoxin ; DNA Replication - genetics ; Endotoxins - genetics ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression - genetics ; genes ; Genetic engineering ; Genetic technics ; genetic vectors ; Genetic Vectors - genetics ; Hemolysin Proteins ; Hydroxylamine ; hydroxylamine mutagenesis ; Hydroxylamines ; Life Sciences ; Methods. Procedures. Technologies ; Mutagenesis ; Plasmid copy number ; plasmids ; Plasmids - genetics ; recombinant DNA ; Recombination, Genetic - genetics ; replication region ; segregational stability ; thuringiensin ; vectors ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; δ-endotoxins
É parte de: Gene, 1991-12, Vol.108 (1), p.115-119
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: The replication region of the Bacillus thuringiensis plasmid, pHT1030, was treated with hydroxylamine. Various copy-number mutants were selected and subsequently used to construct shuttle vectors with multiple cloning sites. These recombinant plasmids are very stable and allowed the cloning of a δ-endotoxin-encoding gene in B. thuringiensis. Comparison between gene expression level and vector copy-number indicated that a plateau in δ-endotoxin production is reached with a copy-number of about fifteen per equivalent chromosome.
Editor: Lausanne: Elsevier B.V
Idioma: Inglês

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Construction of cloning vectors for Bacillus thuringiensis

Arantes, Olivia ; Lereclus, Didier

Lausanne: Elsevier B.V

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