skip to main content
Idioma:
Primo Search
Clear Search Box
Search in: Busca Geral
Busca Avançada
Busca por Índices

Abnormal expression of H-Ras induces S-phase arrest and mitotic catastrophe in human T-lymphocyte leukemia

Zamora Dominguez, Jorge Antonio ; Olarte Carrillo, Irma ; Ruiz Ramos, Rubén ; Ramos-Peñafiel, Christian Omar ; Jiménez Zamudio, Luis ; García Latorre, Ethel ; Centeno Cruz, Federico ; Martínez Tovar, Adolfo

Blood Research, 2023, 58(1), , pp.20-27 [Periódico revisado por pares]

Korea (South): Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis

Texto completo disponível

Citações Citado por
  • Título:
    Abnormal expression of H-Ras induces S-phase arrest and mitotic catastrophe in human T-lymphocyte leukemia
  • Autor: Zamora Dominguez, Jorge Antonio ; Olarte Carrillo, Irma ; Ruiz Ramos, Rubén ; Ramos-Peñafiel, Christian Omar ; Jiménez Zamudio, Luis ; García Latorre, Ethel ; Centeno Cruz, Federico ; Martínez Tovar, Adolfo
  • Assuntos: Original ; 병리학
  • É parte de: Blood Research, 2023, 58(1), , pp.20-27
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Leukemia is a neoplasm with high incidence and mortality rates. Mitotic death has been observed in tumor cells treated with chemotherapeutic agents. Ras family proteins participate in the transduction of signals involved in different processes, such as proliferation, differentiation, survival, and paradoxically, initiation of cell death. This study investigated the effect of expression on human T-cell acute lymphoblastic leukemia MOLT-4 cells. Cells were electroporated with either wild-type (Ras ) or oncogenic mutant in codon 12 exon 1 (Ras ) versions of gene and stained for morphological analysis. Cell viability was assessed using trypan blue staining and cell cycle analysis using flow cytometry. gene expression was determined using quantitative real-time reverse transcription polymerase chain reaction. The , ANOVA, and Scheffe tests were used for statistical analysis. Human T-cell acute lymphoblastic leukemia MOLT-4 cells showed nuclear fragmentation and presence of multiple nuclei and micronuclei after transfection with either wt or mutant genes. Cell cycle analysis revealed a statistically significant increase in cells in the S phase when transfected with either wt (83.67%, <0.0005) or mutated (81.79%, <0.0001) genes. Although similar effects for both versions of were found, cells transfected with the mutated version died at 120 h of mitotic catastrophe. Transfection of human T-cell acute lymphoblastic leukemia MOLT-4 cells with either normal or mutated genes induced alterations in morphology, arrest in the S phase, and death by mitotic catastrophe.
  • Editor: Korea (South): Korean Society of Hematology; Korean Society of Blood and Marrow Transplantation; Korean Society of Pediatric Hematology-Oncology; Korean Society on Thrombosis and Hemostasis
  • Idioma: Inglês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.