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Cryptotanshinone attenuates the stemness of non-small cell lung cancer cells via promoting TAZ translocation from nuclear to cytoplasm

Jin, Linling ; Wu, Zhenzhen ; Wang, Yanli ; Zhao, Xin

Chinese medicine, 2020-06, Vol.15 (1), p.66-66, Article 66 [Periódico revisado por pares]

England: BioMed Central Ltd

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Título:
Cryptotanshinone attenuates the stemness of non-small cell lung cancer cells via promoting TAZ translocation from nuclear to cytoplasm
Autor: Jin, Linling ; Wu, Zhenzhen ; Wang, Yanli ; Zhao, Xin
Assuntos: Adjuvant chemotherapy ; Adjuvant treatment ; Analysis ; Apoptosis ; Breast cancer ; Cancer ; Cancer therapies ; Cell cycle ; Cell viability ; Chemotherapy ; Cryptotanshinone ; Cytoplasm ; Development and progression ; Drug resistance ; Drug therapy ; Enzyme inhibitors ; Experiments ; Gene expression ; Genes ; Health aspects ; Herbal medicine ; Hippo ; Lung cancer ; Lung cancer, Non-small cell ; Lung cancer, Small cell ; Non-small cell lung cancer ; Non-small cell lung carcinoma ; Prostate ; Protein-tyrosine kinase ; Ribonucleic acid ; RNA ; Sequence analysis ; Signal transduction ; Small cell lung cancer ; Small cell lung carcinoma ; Spheroids ; Stem cell transplantation ; Stem cells ; Stemness ; TAZ ; Transplantation ; Tyrosine
É parte de: Chinese medicine, 2020-06, Vol.15 (1), p.66-66, Article 66
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Cancer stem cells (CSCs) are regarded as the root of tumor progression, thus representing an anti-cancer therapy through targeting this cell sub-population. Non-small cell lung cancer (NSCLC) CSCs were enriched by non-adherent spheroid formation analysis. Lentivirus infection was used to stably change gene expression. Cell cycle, EdU incorporation, cell apoptosis, cell viability, ALDH1 activity, spheroid formation and in vivo tumor initiation assays were performed to detect the effects of Cryptotanshinone (CT), a traditional Chinese herb medicine, on the stemness of NSCLC cells. RNA-sequencing combined qRT-PCR and western blot analysis were constructed to explore the underlying mechanism contributing to CT-mediated effects. CT could attenuate the stemness of NSCLC CSCs, as evident by the reduced spheroid formation ability, stemness marker expression and ALDH1 activity. Additionally, CT provoked NSCLC CSCs entry into the cell cycle. RNA-sequencing analysis showed that Hippo signaling pathway was highly enriched in NSCLC CSCs with CT treatment. Further experiments disclosed that CT decreased TAZ (a regulatory master of Hippo pathway) expression via promoting its nuclear-cytoplasm translocation in NSCLC CSCs. Also, overexpression of TAZ partially saved the attenuation of CT on the stemness of NSCLC CSCs. Notably, CT enhanced the sensitivity of tyrosine kinase inhibitor (TKI) and chemotherapy in NSCLC CSCs. This work reveals that CT attenuates NSCLC CSC stemness, implying the possibility of CT as an adjuvant therapy for NSCLC.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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Cryptotanshinone attenuates the stemness of non-small cell lung cancer cells via promoting TAZ translocation from nuclear to cytoplasm

Jin, Linling ; Wu, Zhenzhen ; Wang, Yanli ; Zhao, Xin

England: BioMed Central Ltd

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