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AIDS Kaposi Sarcoma-Derived Cells Produce and Respond to Interleukin 6

Miles, Steven A. ; Rezai, Ahmad R. ; Salazar-Gonzalez, Jesus F. ; Meyden, Meta Vander ; Stevens, Ronald H. ; Logan, Diane M. ; Mitsuyasu, Ronald T. ; Taga, Tetsuya ; Hirano, Toshio ; Kishimoto, Tadamitsu ; Martinez-Maza, Otoniel

Proceedings of the National Academy of Sciences - PNAS, 1990-06, Vol.87 (11), p.4068-4072 [Periódico revisado por pares]

Washington, DC: National Academy of Sciences of the United States of America

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Título:
AIDS Kaposi Sarcoma-Derived Cells Produce and Respond to Interleukin 6
Autor: Miles, Steven A. ; Rezai, Ahmad R. ; Salazar-Gonzalez, Jesus F. ; Meyden, Meta Vander ; Stevens, Ronald H. ; Logan, Diane M. ; Mitsuyasu, Ronald T. ; Taga, Tetsuya ; Hirano, Toshio ; Kishimoto, Tadamitsu ; Martinez-Maza, Otoniel
Assuntos: 550201 - Biochemistry- Tracer Techniques ; Acquired Immunodeficiency Syndrome - pathology ; Acquired Immunodeficiency Syndrome - physiopathology ; AIDS ; AIDS/HIV ; ANIMAL CELLS ; ANIMAL TISSUES ; AZINES ; B lymphocytes ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; BIOASSAY ; Biological and medical sciences ; BIOLOGICAL MATERIALS ; BIOSYNTHESIS ; BLOOD ; BLOOD CELLS ; Blotting, Northern ; BODY ; BODY FLUIDS ; Cell growth ; Cell lines ; CELL PROLIFERATION ; Cultured cells ; DAYS LIVING RADIOISOTOPES ; DISEASES ; DNA ; DNA HYBRIDIZATION ; Endothelial cells ; ENDOTHELIUM ; Epithelial cells ; Gene Expression ; GROWTH FACTORS ; Growth Substances - physiology ; HETEROCYCLIC COMPOUNDS ; Humans ; HYBRIDIZATION ; HYDROGEN COMPOUNDS ; IMMUNE SYSTEM DISEASES ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; In Vitro Techniques ; INFECTIOUS DISEASES ; Interleukin-6 - genetics ; Interleukin-6 - metabolism ; Interleukin-6 - pharmacology ; ISOTOPES ; Kaposi sarcoma ; LEUKOCYTES ; LIGHT NUCLEI ; LYMPHOKINES ; MATERIALS ; Medical sciences ; MEMBRANE PROTEINS ; MESSENGER-RNA ; MITOGENS ; MONOCYTES ; NEOPLASMS ; NUCLEI ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ODD-ODD NUCLEI ; OLIGONUCLEOTIDES ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; PATHOGENESIS ; PATIENTS ; PHOSPHORUS 32 ; PHOSPHORUS ISOTOPES ; PROTEINS ; PYRIMIDINES ; RADIOISOTOPES ; RECEPTORS ; Receptors, Immunologic - physiology ; Receptors, Interleukin-6 ; RECOMBINANT DNA ; Recombinant Proteins ; RIBOSIDES ; RNA ; RNA, Antisense ; Sarcoma, Kaposi - pathology ; Sarcoma, Kaposi - physiopathology ; SARCOMAS ; SYNTHESIS ; T lymphocytes ; THYMIDINE ; TISSUES ; TRITIUM COMPOUNDS ; TUMOR CELLS ; Tumor Cells, Cultured ; VIRAL DISEASES
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1990-06, Vol.87 (11), p.4068-4072
Notas: ObjectType-Article-2
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Descrição: Cell lines derived from Kaposi sarcoma lesions of patients with AIDS (AIDS-KS cells) produce several cytokines, including an endothelial cell growth factor, interleukin 1β, and basic fibroblast growth factor. Since exposure to human immunodeficiency virus increases interleukin 6 (IL-6) production in monocytes and endothelial cells produce IL-6, we examined IL-6 expression and response in AIDS-KS cell lines and IL-6 expression in AIDS Kaposi sarcoma tissue. The AIDS-KS cell lines (N521J and EKS3) secreted large amounts of immunoreactive and biologically active IL-6. We found both IL-6 and IL-6 receptor (IL-6-R) RNA by slot blot hybridization analysis of AIDS-KS cells. The IL-6-R was functional, as [3H]thymidine incorporation by AIDS-KS cells increased significantly after exposure to human recombinant IL-6 (hr IL-6) at >10 units/ml. When AIDS-KS cells (EKS3) were exposed to IL-6 antisense oligonucleotide, cellular proliferation decreased by nearly two-thirds, with a corresponding decrease in the production of IL-6. The decrease from IL-6 antisense in AIDS-KS cell proliferation was reversed by the addition of hrIL-6. We confirmed that AIDS-KS cells produced IL-6 in vivo by preparing RNA and tissue sections from involved and uninvolved skin from a patient with AIDS Kaposi sarcoma. We detected immunoreactive IL-6 in the involved tumor areas and to a lesser extent in the surrounding normal epidermis. Slot blot hybridization showed a great excess of IL-6 and IL-6-R RNA in involved skin compared to uninvolved skin. These results show that both IL-6 and IL-6-R are produced by AIDS-KS cells and that IL-6 is required for optimal AIDS-KS cell proliferation, and they suggest that IL-6 is an autocrine growth factor for AIDS-KS cells.
Editor: Washington, DC: National Academy of Sciences of the United States of America
Idioma: Inglês

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AIDS Kaposi Sarcoma-Derived Cells Produce and Respond to Interleukin 6

Miles, Steven A. ; Rezai, Ahmad R. ; Salazar-Gonzalez, Jesus F. ; Meyden, Meta Vander ; Stevens, Ronald H. ; Logan, Diane M. ; Mitsuyasu, Ronald T. ; Taga, Tetsuya ; Hirano, Toshio ; Kishimoto, Tadamitsu ; Martinez-Maza, Otoniel

Washington, DC: National Academy of Sciences of the United States of America

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