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Association of MiR-126 with soluble mesothelin-related peptides, a marker for malignant mesothelioma

Santarelli, Lory ; Strafella, Elisabetta ; Staffolani, Sara ; Amati, Monica ; Emanuelli, Monica ; Sartini, Davide ; Pozzi, Valentina ; Carbonari, Damiano ; Bracci, Massimo ; Pignotti, Elettra ; Mazzanti, Paola ; Sabbatini, Armando ; Ranaldi, Renzo ; Gasparini, Stefano ; Neuzil, Jiri ; Tomasetti, Marco Pandey, Siyaram

PloS one, 2011-04, Vol.6 (4), p.e18232 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Association of MiR-126 with soluble mesothelin-related peptides, a marker for malignant mesothelioma
Autor: Santarelli, Lory ; Strafella, Elisabetta ; Staffolani, Sara ; Amati, Monica ; Emanuelli, Monica ; Sartini, Davide ; Pozzi, Valentina ; Carbonari, Damiano ; Bracci, Massimo ; Pignotti, Elettra ; Mazzanti, Paola ; Sabbatini, Armando ; Ranaldi, Renzo ; Gasparini, Stefano ; Neuzil, Jiri ; Tomasetti, Marco
Pandey, Siyaram
Assuntos: Aged ; Aged, 80 and over ; Angiogenesis ; Asbestos ; Asbestos - adverse effects ; Biochemistry ; Bioindicators ; Biology ; Biomarkers ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - chemistry ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Biopsy ; Cancer therapies ; Case-Control Studies ; Chromosomes ; Deregulation ; Disease ; Down-Regulation - drug effects ; Early Detection of Cancer ; Environmental Exposure - adverse effects ; Epigenetics ; Epithelium - drug effects ; Epithelium - metabolism ; Exposure ; Female ; Formaldehyde ; Gene Expression Regulation, Neoplastic - drug effects ; GPI-Linked Proteins - chemistry ; GPI-Linked Proteins - metabolism ; Hospitals ; Humans ; Hypoxia ; Identification ; Lung cancer ; Male ; Medical diagnosis ; Medical prognosis ; Medicine ; Mesothelioma ; Mesothelioma - chemically induced ; Mesothelioma - diagnosis ; Mesothelioma - metabolism ; Mesothelioma - pathology ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; Paraffin ; Pathology ; Patients ; Peptides ; Peptides - chemistry ; Peptides - metabolism ; Polymerase chain reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Serum levels ; Solubility ; Thoracic surgery ; Tissues ; Tumors ; Vascular endothelial growth factor
É parte de: PloS one, 2011-04, Vol.6 (4), p.e18232
Notas: Conceived and designed the experiments: LS MA MT. Performed the experiments: ES SS DS VP DC. Analyzed the data: MB EP JN MT. Contributed reagents/materials/analysis tools: LS MA ME. Wrote the paper: MT JN. Patient management and sample collection: PM AS RR SG. Quantitative real-time PCR assay and data analysis: ME.
Descrição: Improved detection methods for diagnosis of malignant pleural mesothelioma (MPM) are essential for early and reliable detection as well as treatment. Since recent data point to abnormal levels of microRNAs (miRNAs) in tumors, we hypothesized that a profile of deregulated miRNAs may be a marker of MPM and that the levels of specific miRNAs may be used for monitoring its progress. miRNAs isolated from fresh-frozen biopsies of MPM patients were tested for the expression of 88 types of miRNA involved in cancerogenesis. Most of the tested miRNAs were downregulated in the malignant tissues compared with the normal tissues. Of eight significantly downregulated, three miRNAs were assayed in cancerous tissue and adjacent non-cancerous tissue sample pairs collected from 27 formalin-fixed, paraffin-embedded MPM tissues by quantitative RT-PCR. Among the miRNAs tested, only miR-126 significantly remained downregulated in the malignant tissues. Furthermore, the performance of the selected miR-126 as biomarker was evaluated in serum samples of asbestos-exposed subjects and MPM patients and compared with controls. MiR-126 was not affected by asbestos exposure, whereas it was found strongly associated with VEGF serum levels. Levels of miR-126 in serum, and its levels in patients' serum in association with a specific marker of MPM, SMRPs, correlate with subjects at high risk to develop MPM. We propose miR-126, in association with SMRPs, as a marker for early detection of MPM. The identification of tumor biomarkers used alone or, in particular, in combination could greatly facilitate the surveillance procedure for cohorts of subjects exposed to asbestos.
Editor: United States: Public Library of Science
Idioma: Inglês

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Association of MiR-126 with soluble mesothelin-related peptides, a marker for malignant mesothelioma

United States: Public Library of Science

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