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A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Moen, Scott T ; Hatcher, Christopher L ; Singh, Anup K Chalmers, Jeffrey

PloS one, 2016-04, Vol.11 (4), p.e0153137-e0153137 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections
Autor: Moen, Scott T ; Hatcher, Christopher L ; Singh, Anup K
Chalmers, Jeffrey
Assuntos: BASIC BIOLOGICAL SCIENCES ; Biological samples ; Biology and Life Sciences ; Blood ; Blood cells ; Blood Cells - cytology ; Cell Separation - instrumentation ; Cell Separation - methods ; cell staining ; Centrifugation ; Centrifugation, Density Gradient - instrumentation ; Centrifugation, Density Gradient - methods ; Contact angle ; density gradient centrifugation ; Density gradients ; Engineering and Technology ; Erythrocytes ; Humans ; Laboratories ; Lasers ; Leukemia ; Leukocytes ; Medical research ; Medicine and Health Sciences ; Methods ; Microfluidic Analytical Techniques - instrumentation ; Microfluidic Analytical Techniques - methods ; Microfluidics ; Microscopy ; OTHER INSTRUMENTATION ; red blood cells ; Research and Analysis Methods ; Sensors ; Separation ; Separation (Technology) ; white blood cells ; Wright-Giemsa staining
É parte de: PloS one, 2016-04, Vol.11 (4), p.e0153137-e0153137
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
AC04-94AL85000; R01AI0988530
USDOE National Nuclear Security Administration (NNSA)
Conceived and designed the experiments: STM. Performed the experiments: STM. Analyzed the data: STM CLH AKS. Contributed reagents/materials/analysis tools: STM. Wrote the paper: STM CLH AKS.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.
Editor: United States: Public Library of Science
Idioma: Inglês

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A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Moen, Scott T ; Hatcher, Christopher L ; Singh, Anup K Chalmers, Jeffrey

United States: Public Library of Science

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