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The cellular phenotype of cytoplasmic incompatibility in Culex pipiens in the light of cidB diversity

Bonneau, Manon ; Landmann, Frédéric ; Labbé, Pierrick ; Justy, Fabienne ; Weill, Mylène ; Sicard, Mathieu McGraw, Elizabeth Ann

PLoS pathogens, 2018-10, Vol.14 (10), p.e1007364-e1007364 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
The cellular phenotype of cytoplasmic incompatibility in Culex pipiens in the light of cidB diversity
Autor: Bonneau, Manon ; Landmann, Frédéric ; Labbé, Pierrick ; Justy, Fabienne ; Weill, Mylène ; Sicard, Mathieu
McGraw, Elizabeth Ann
Assuntos: Animal biology ; Animal genetics ; Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - microbiology ; Antidotes ; Arthropods ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biology and Life Sciences ; Chromatin ; Culex - genetics ; Culex - microbiology ; Culex pipiens ; Cytoplasm - microbiology ; Cytoplasm - pathology ; Cytoplasmic incompatibility ; Death ; Defects ; Deoxyribonucleic acid ; Development Biology ; Diptera ; DNA ; Drosophila melanogaster ; Drosophila melanogaster - genetics ; Drosophila melanogaster - microbiology ; Eggs ; Embryology and Organogenesis ; Embryos ; Evolution ; Female ; Females ; Genetic aspects ; Genetics ; Genomes ; Genomics ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - pathology ; Health aspects ; Host Specificity ; Host-parasite relationships ; Incompatibility ; Insecticides ; Insects ; Invertebrate Zoology ; Life Sciences ; Male ; Males ; Medicine and Health Sciences ; Mortality ; Mosquitoes ; Phenotype ; Phenotypes ; Physical Sciences ; Polymorphism, Genetic ; Reproductive Biology ; Research and Analysis Methods ; Sexual reproduction ; Sperm ; Strains (organisms) ; Symbiosis ; Toxins ; Wolbachia ; Wolbachia - physiology
É parte de: PLoS pathogens, 2018-10, Vol.14 (10), p.e1007364-e1007364
Notas: new_version
ObjectType-Article-1
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PMCID: PMC6201942
The authors have declared that no competing interests exist.
Descrição: Wolbachia are maternally inherited endosymbiotic bacteria, widespread among arthropods thanks to host reproductive manipulations that increase their prevalence into host populations. The most commonly observed manipulation is cytoplasmic incompatibility (CI). CI leads to embryonic death in crosses between i) infected males and uninfected females and ii) individuals infected with incompatible Wolbachia strains. CI can be conceptualized as a toxin-antidote system where a toxin deposited by Wolbachia in the sperm would induce embryonic death unless countered by an antidote produced by Wolbachia present in the eggs. In Drosophila melanogaster, transgenic expression of Wolbachia effector cidB revealed its function of CI-inducing toxin. Moreover in Culex pipiens, the diversity of cidB variants present in wPip strains accounts for the diversity in crossing-types. We conducted cytological analyses to determine the CI mechanisms that lead to embryonic death in C. pipiens, and assess whether diversity in crossing-types could be based on variations in these mechanisms. We revealed that paternal chromatin condensation and segregation defects during the first embryonic division are always responsible for embryonic death. The strongest observed defects lead to an exclusion of the paternal chromatin from the first zygotic division, resulting in haploid embryos unable to hatch. The proportion of unhatched haploid embryos, developing with only maternal chromatin, which reflects the frequency of strong defects can be considered as a proxy of CI intensity at the cellular level. We thus studied the putative effect of variations in crossing types and cidB diversification on CI defects intensity. Incompatible crosses involving distinct wPip strains revealed that CI defects intensity depends on the Wolbachia strains hosted by the males and is linked to the diversity of cidB genes harbored in their genomes. These results support that, additionally to its implication in C. pipiens crossing type variability, cidB diversification also influences the strength of CI embryonic defects.
Editor: United States: Public Library of Science
Idioma: Inglês

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The cellular phenotype of cytoplasmic incompatibility in Culex pipiens in the light of cidB diversity

Bonneau, Manon ; Landmann, Frédéric ; Labbé, Pierrick ; Justy, Fabienne ; Weill, Mylène ; Sicard, Mathieu McGraw, Elizabeth Ann

United States: Public Library of Science

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