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Chronic cannabidiol (CBD) administration induces anticonvulsant and antiepileptogenic effects in a genetic model of epilepsy

Lazarini-Lopes, Willian ; Do Val-da Silva, Raquel A. ; da Silva-Júnior, Rui M.P. ; Silva-Cardoso, Gleice K. ; Leite-Panissi, Christie R.A. ; Leite, João P. ; Garcia-Cairasco, Norberto

Epilepsy & behavior, 2021-06, Vol.119, p.107962-107962, Article 107962 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Chronic cannabidiol (CBD) administration induces anticonvulsant and antiepileptogenic effects in a genetic model of epilepsy
  • Autor: Lazarini-Lopes, Willian ; Do Val-da Silva, Raquel A. ; da Silva-Júnior, Rui M.P. ; Silva-Cardoso, Gleice K. ; Leite-Panissi, Christie R.A. ; Leite, João P. ; Garcia-Cairasco, Norberto
  • Assuntos: Anticonvulsant ; Cannabidiol ; CB1 receptors ; Epilepsy ; Neuronal activity ; Preclinical model
  • É parte de: Epilepsy & behavior, 2021-06, Vol.119, p.107962-107962, Article 107962
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: •Chronic cannabidiol (CBD) administration induced anticonvulsant and antiepileptogenic effects.•CBD prevented chronic neuronal hyperactivity induced by chronic seizures.•CBD attenuated brainstem seizures and prevented the development of limbic seizures.•Chronic CBD prevented neuroplastic changes in CB1R induced by chronic seizures.•Neuroethological analysis supports chronic CBD protective effects for epilepsy. Cannabidiol (CBD) is a marijuana compound implicated in epilepsy treatment in animal models and pharmacoresistant patients. However, little is known about chronic CBD administration’s effects in chronic models of seizures, especially regarding its potential antiepileptogenic effects. In the present study, we combined a genetic model of epilepsy (the Wistar Audiogenic Rat strain - WARs), a chronic protocol of seizures (the audiogenic kindling - AuK), quantitative and sequential behavioral analysis (neuroethology), and microscopy imaging to analyze the effects of chronic CBD administration in a genetic model of epilepsy. The acute audiogenic seizure is characterized by tonic-clonic seizures and intense brainstem activity. However, during the AuK WARs can develop limbic seizures associated with the recruitment of forebrain and limbic structures. Here, chronic CBD administration, twice a day, attenuated brainstem, tonic-clonic seizures, prevented limbic recruitment, and suppressed limbic (kindled) seizures, suggesting CBD antiepileptogenic effects. Additionally, CBD prevented chronic neuronal hyperactivity, suppressing FosB immunostaining in the brainstem (inferior colliculus and periaqueductal gray matter) and forebrain (basolateral amygdala nucleus and piriform cortex), structures associated with tonic-clonic and limbic seizures, respectively. Chronic seizures increased cannabinoid receptors type 1 (CB1R) immunostaining in the hippocampus and the BLA, while CBD administration prevented changes in CB1R expression induced by the AuK. The neuroethological analysis provided details about CBD’s protective effects against brainstem and limbic seizures associated with FosB expression. Our results strongly suggest chronic CBD anticonvulsant and antiepileptogenic effects associated with reduced chronic neuronal activity and modulation of CB1R expression. We also support the chronic use of CBD for epilepsies treatments.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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