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Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells

Stefanowicz-Hajduk, Justyna ; Bartoszewski, Rafal ; Bartoszewska, Sylwia ; Kochan, Kinga ; Adamska, Anna ; Kosiński, Igor ; Ochocka, J Renata Zhivotovsky, Boris

PloS one, 2015-08, Vol.10 (8), p.e0135993-e0135993 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells
Autor: Stefanowicz-Hajduk, Justyna ; Bartoszewski, Rafal ; Bartoszewska, Sylwia ; Kochan, Kinga ; Adamska, Anna ; Kosiński, Igor ; Ochocka, J Renata
Zhivotovsky, Boris
Assuntos: Adenocarcinoma ; Analysis ; Anticancer properties ; Antineoplastic Agents, Phytogenic - isolation & purification ; Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis ; Apoptosis - drug effects ; BH3 Interacting Domain Death Agonist Protein - agonists ; BH3 Interacting Domain Death Agonist Protein - genetics ; BH3 Interacting Domain Death Agonist Protein - metabolism ; Biological properties ; Biology ; Cancer therapies ; Caspase ; Caspase 8 - genetics ; Caspase 8 - metabolism ; Caspase-8 ; Cell cycle ; Cell death ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cervical cancer ; Cervix ; Cytotoxicity ; Drug therapy ; Experiments ; FADD protein ; Fas-Associated Death Domain Protein - agonists ; Fas-Associated Death Domain Protein - genetics ; Fas-Associated Death Domain Protein - metabolism ; Female ; Flow cytometry ; Formulations ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; HeLa Cells ; Humans ; Inhibitory Concentration 50 ; Liliaceae - chemistry ; Medical research ; Membrane potential ; Membrane Potential, Mitochondrial - drug effects ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Mitochondria - pathology ; Molecular chains ; Molecular modelling ; Penicillin ; Pharmaceuticals ; Pharmacology ; Physiological aspects ; Rhizome - chemistry ; Rhizomes ; Saponins ; Saponins - isolation & purification ; Saponins - pharmacology ; Signal Transduction ; Tumor cells
É parte de: PloS one, 2015-08, Vol.10 (8), p.e0135993-e0135993
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: JSH RB JRO. Performed the experiments: JSH RB SB KK AA IK. Analyzed the data: JSH RB. Wrote the paper: JSH.
Descrição: Pennogenyl saponins are the active compounds of large number of plant species and consequently many polyherbal formulations. Hence, great interest has been shown in their characterization and in the investigation of their pharmacological and biological properties, especially anticancer. This present study reports on the evaluation of cytotoxic effects and explanation of the molecular mechanisms of action of the two pennogenyl saponins (PS 1 and PS 2) isolated from Paris quadrifolia L. rhizomes on human cervical adenocarcinoma cell line HeLa. To determine the viability of the cells treated with the compounds we used real-time cell proliferation analysis and found that the pennogenyl saponins PS 1 and PS 2 strongly inhibited the tumor cells growth with IC50 values of 1.11 ± 0.04 μg/ml and 0.87 ± 0.05 μg/ml, respectively. The flow cytometry analysis indicated that the two compounds induced apoptosis in a dose-dependent manner and decreased mitochondrial membrane potential in HeLa cells in the early stage of apoptosis. Quantitative PCR and Western Blot analysis showed that the two saponins significantly increased mRNA expression of FADD and BID as well as induced caspase-8 via increased of procaspase-8 processing in the treated cells. The results of this study suggest that both the extrinsic death receptor and intrinsic mitochondrial pathways are involved in the programmed cell death.
Editor: United States: Public Library of Science
Idioma: Inglês

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Pennogenyl Saponins from Paris quadrifolia L. Induce Extrinsic and Intrinsic Pathway of Apoptosis in Human Cervical Cancer HeLa Cells

Stefanowicz-Hajduk, Justyna ; Bartoszewski, Rafal ; Bartoszewska, Sylwia ; Kochan, Kinga ; Adamska, Anna ; Kosiński, Igor ; Ochocka, J Renata Zhivotovsky, Boris

United States: Public Library of Science

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