Idioma:

Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells studied by in vitro translation

Bludau, H. ; Kopun, M. ; Werner, D.

Experimental cell research, 1986-07, Vol.165 (1), p.269-282 [Periódico revisado por pares]

Orlando, FL: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells studied by in vitro translation
Autor: Bludau, H. ; Kopun, M. ; Werner, D.
Assuntos: Animals ; Antibodies, Monoclonal ; Antigens, Nuclear ; Biological and medical sciences ; Carcinoma, Ehrlich Tumor - metabolism ; Cell Cycle ; Cell cycle, cell proliferation ; Cell Fractionation ; Cell physiology ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Molecular and cellular biology ; Nucleoproteins - biosynthesis ; Poly A - metabolism ; Protein Biosynthesis ; RNA - metabolism ; RNA, Messenger - metabolism
É parte de: Experimental cell research, 1986-07, Vol.165 (1), p.269-282
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: A combination of methods was used to study the cell cycle-dependent expression of nuclear matrix proteins of Ehrlich ascites cells: 1. 1. Separation of asynchronous cells growing in vivo into fractions of G1-, S- and G2-phase cells by centrifugal elutriation with less than 10% cross-contamination. 2. 2. Isolation of poly(A +) RNA populations from total cytoplasmic RNA by affinity chromatography on messenger affinity paper (mAP). 3. 3. In vitro translation of poly(A +) RNA from asynchronous and phase synchronous cells. 4. 4. Immunoprecipitation of in vitro synthesized nuclear matrix proteins by a monoclonal antibody with anti-lamin specificity (PKB8) and by a polyspecific anti-nuclear matrix serum (AMS5) followed by analysis of immunoprecipitated materials on SDS-polyacrylamide gels. The results indicate that mRNAs for nuclear matrix-associated proteins including the lamins B and C are either exclusively or at least predominantly present in the cytoplasm of cells in S phase suggesting a high rate of in vivo synthesis of these proteins during S phase. This is consistent with an anticipated biological function of the nuclear matrix which is considered to organize parental and newly synthesized DNA in higher order structures.
Editor: Orlando, FL: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Cell cycle-dependent expression of nuclear matrix proteins of ehrlich ascites cells studied by in vitro translation

Bludau, H. ; Kopun, M. ; Werner, D.

Orlando, FL: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.