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No significant overreplication occurs in Ehrlich ascites cells during and after reversal of hypoxia

Probst, Hans ; Riedinger, Hans-Jörg ; Gekeler, Volker

Experimental cell research, 1989-02, Vol.180 (2), p.563-568 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
No significant overreplication occurs in Ehrlich ascites cells during and after reversal of hypoxia
Autor: Probst, Hans ; Riedinger, Hans-Jörg ; Gekeler, Volker
Assuntos: Animals ; Bromodeoxyuridine ; Carcinoma, Ehrlich Tumor - genetics ; Carcinoma, Ehrlich Tumor - metabolism ; Carcinoma, Ehrlich Tumor - pathology ; Demecolcine - pharmacology ; DNA Replication - drug effects ; Hypoxia - genetics ; Hypoxia - metabolism ; Hypoxia - pathology ; Interphase ; Mice
É parte de: Experimental cell research, 1989-02, Vol.180 (2), p.563-568
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SourceType-Scholarly Journals-1
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content type line 23
Descrição: Labeling with bromodeoxyuridine and analysis by isopycnic banding for emergence of DNA-helices density-labeled in both strands (HH-DNA) were used to examine whether transient controlled hypoxia induces significant overreplication in the DNA of cultured Ehrlich ascites cells within a single cell cycle. Diverse situations of the hypoxic period (4–8 h) within the BrdUrd labeling time (8–16 h) were tested. If traversal of more than one complete cell cycle by very fast cycling individual cells was avoided by using short BrdUrd labeling periods or by addition of colcemid, HH-DNA was never detected. This indicates that hypoxia does not induce significant overreplication under the conditions chosen and, in particular, that the burst of replicon initiations occurring after reoxygenation of hypoxic cells principally is not due to overreplication.
Editor: United States: Elsevier Inc
Idioma: Inglês

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No significant overreplication occurs in Ehrlich ascites cells during and after reversal of hypoxia

Probst, Hans ; Riedinger, Hans-Jörg ; Gekeler, Volker

United States: Elsevier Inc

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