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A functional proteomic method for biomarker discovery

Reynolds, Fred ; Panneer, Nivedha ; Tutino, Christopher M ; Wu, Michael ; Skrabal, William R ; Moskaluk, Christopher ; Kelly, Kimberly A Pant, Harish

PloS one, 2011-07, Vol.6 (7), p.e22471-e22471 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A functional proteomic method for biomarker discovery
Autor: Reynolds, Fred ; Panneer, Nivedha ; Tutino, Christopher M ; Wu, Michael ; Skrabal, William R ; Moskaluk, Christopher ; Kelly, Kimberly A
Pant, Harish
Assuntos: Affinity ; Amino Acid Sequence ; Analysis ; Apoptosis ; Biochemistry ; Biology ; Biomarkers ; Biomarkers - metabolism ; Biomarkers, Tumor - metabolism ; Biomedical engineering ; Combinatorial analysis ; Combinatorial chemistry ; Deoxyribonucleic acid ; DNA ; DNA probes ; Engineering ; Gene sequencing ; Genetic transcription ; Genomes ; Health screening ; Humans ; Identification methods ; Ligands ; Lymphoma ; Mass Spectrometry ; Medical screening ; Medicine ; Methods ; Molecular Sequence Data ; Pancreatic cancer ; Peptide Library ; Peptides ; Peptides - chemistry ; Peptides - metabolism ; Phage display ; Phages ; Pharmacogenetics ; Pharmacogenomics ; Post-transcription ; Precision medicine ; Probes ; Protein expression ; Proteins ; Proteomics ; Proteomics - methods ; Reproducibility of Results ; Ribonucleic acid ; RNA ; Target recognition ; Tissue Array Analysis ; Tissues ; Transcription ; Transcription (Genetics) ; Tumors
É parte de: PloS one, 2011-07, Vol.6 (7), p.e22471-e22471
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: FR KAK. Performed the experiments: FR NP CMT MW WRS CM. Analyzed the data: FR KAK CM. Contributed reagents/materials/analysis tools: CM. Wrote the paper: FR KAK.
Descrição: The sequencing of the human genome holds out the hope for personalized medicine, but it is clear that analysis of DNA or RNA content alone is not sufficient to understand most disease processes. Proteomic strategies that allow unbiased identification of proteins and their post-transcriptional and -translation modifications are an essential complement to genomic strategies. However, the enormity of the proteome and limitations in proteomic methods make it difficult to determine the targets that are particularly relevant to human disease. Methods are therefore needed that allow rational identification of targets based on function and relevance to disease. Screening methodologies such as phage display, SELEX, and small-molecule combinatorial chemistry have been widely used to discover specific ligands for cells or tissues of interest, such as tumors. Those ligands can be used in turn as affinity probes to identify their cognate molecular targets when they are not known in advance. Here we report an easy, robust and generally applicable approach in which phage particles bearing cell- or tissue-specific peptides serve directly as the affinity probes for their molecular targets. For proof of principle, the method successfully identified molecular binding partners, three of them novel, for 15 peptides specific for pancreatic cancer.
Editor: United States: Public Library of Science
Idioma: Inglês

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A functional proteomic method for biomarker discovery

Reynolds, Fred ; Panneer, Nivedha ; Tutino, Christopher M ; Wu, Michael ; Skrabal, William R ; Moskaluk, Christopher ; Kelly, Kimberly A Pant, Harish

United States: Public Library of Science

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