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Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

Lacerda Pigosso, Laurine ; Baeza, Lilian Cristiane ; Vieira Tomazett, Mariana ; Batista Rodrigues Faleiro, Mariana ; Brianezi Dignani de Moura, Veridiana Maria ; Melo Bailão, Alexandre ; Borges, Clayton Luiz ; Alves Parente Rocha, Juliana ; Rocha Fernandes, Gabriel ; Gauthier, Gregory M. ; Soares, Celia Maria de Almeida

Virulence, 2017-10, Vol.8 (7), p.1417-1434 [Periódico revisado por pares]

United States: Taylor & Francis

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Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection
Autor: Lacerda Pigosso, Laurine ; Baeza, Lilian Cristiane ; Vieira Tomazett, Mariana ; Batista Rodrigues Faleiro, Mariana ; Brianezi Dignani de Moura, Veridiana Maria ; Melo Bailão, Alexandre ; Borges, Clayton Luiz ; Alves Parente Rocha, Juliana ; Rocha Fernandes, Gabriel ; Gauthier, Gregory M. ; Soares, Celia Maria de Almeida
Assuntos: Animals ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Gene Expression Regulation, Fungal ; Humans ; Male ; metabolic adaptation ; Mice ; Mice, Inbred BALB C ; Oxidative Stress ; Paracoccidioides - enzymology ; Paracoccidioides - genetics ; Paracoccidioides - physiology ; Paracoccidioides brasiliensis ; Paracoccidioidomycosis - microbiology ; Protein Transport ; proteome ; Proteomics ; Research Paper ; Serine Proteases - genetics ; Serine Proteases - metabolism ; serine proteinase ; transcriptome
É parte de: Virulence, 2017-10, Vol.8 (7), p.1417-1434
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SourceType-Scholarly Journals-1
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Supplemental data for this article can be accessed on the publisher's website.
Descrição: Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MS E . In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins. Integration of transcriptional and proteomic data indicated that early in infection (6 h), P. brasiliensis yeast cells underwent a shift in metabolism from glycolysis to β-oxidation, upregulated detoxifying enzymes to defend against oxidative stress, and repressed cell wall biosynthesis. Bioinformatics and functional analyses also demonstrated that a serine proteinase was upregulated and secreted in vivo. To our knowledge this is the first study depicting transcriptional and proteomic data of P. brasiliensis yeast cells upon 6 h post-infection of mouse lung.
Editor: United States: Taylor & Francis
Idioma: Inglês

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Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

United States: Taylor & Francis

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