Idioma:

Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection

Nesa, Jannatun ; Jana, Swapan Kumar ; Sadat, Abdul ; Biswas, Kinkar ; Kati, Ahmet ; Kaya, Ozge ; Mondal, Rittick ; Dam, Paulami ; Thakur, Mintu ; Kumar, Anoop ; Hossain, Maidul ; Lima, Lucas R ; Rezende, Samilla B ; Bhattacharjya, Debjoy ; Gangopadhyay, Debnirmalya ; Ghorai, Suvankar ; Altuntas, Sevde ; Panda, Amiya Kumar ; Chakrabarti, Pinak ; Swarnakar, Shambhu ; Chakraborty, Joydeep ; Yilmaz, Berfin ; Macedo, Maria L R ; Franco, Octávio L ; Cardoso, Marlon H ; Mandal, Amit Kumar

Scientific reports, 2022-09, Vol.12 (1), p.15493-19, Article 15493 [Revista revisada por pares]

England: Nature Publishing Group

Texto completo disponible

Citas Citado por

Acciones

Título:
Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection
Autor: Nesa, Jannatun ; Jana, Swapan Kumar ; Sadat, Abdul ; Biswas, Kinkar ; Kati, Ahmet ; Kaya, Ozge ; Mondal, Rittick ; Dam, Paulami ; Thakur, Mintu ; Kumar, Anoop ; Hossain, Maidul ; Lima, Lucas R ; Rezende, Samilla B ; Bhattacharjya, Debjoy ; Gangopadhyay, Debnirmalya ; Ghorai, Suvankar ; Altuntas, Sevde ; Panda, Amiya Kumar ; Chakrabarti, Pinak ; Swarnakar, Shambhu ; Chakraborty, Joydeep ; Yilmaz, Berfin ; Macedo, Maria L R ; Franco, Octávio L ; Cardoso, Marlon H ; Mandal, Amit Kumar
Materias: Acetic acid ; Animals ; Ant Venoms ; Anti-Bacterial Agents - pharmacology ; Anti-Infective Agents ; Antibacterial activity ; Antibiotic resistance ; Antimicrobial agents ; Antimicrobial peptides ; Bombyx ; Bombyx mori ; Cell lines ; Cell viability ; Erythrocytes ; Fibroblasts ; Fourier transforms ; Hemolymph ; High-performance liquid chromatography ; Humans ; Immune system ; Innate immunity ; Inoculum ; Ionization ; Ions ; Liquid chromatography ; Methanol ; Peptides ; Peptides - chemistry ; Pseudomonas aeruginosa ; Pseudomonas Infections - drug therapy ; Scanning electron microscopy ; Venom ; Water
Es parte de: Scientific reports, 2022-09, Vol.12 (1), p.15493-19, Article 15493
Descripción: The main effectors in the innate immune system of Bombyx mori L. are antimicrobial peptides (AMPs). Here, we infected B. mori with varied inoculum sizes of Pseudomonas aeruginosa ATCC 25668 cells to investigate changes in morpho-anatomical responses, physiological processes and AMP production. Ultraviolet-visible spectra revealed a sharp change in λ from 278 to 285 nm (bathochromic shift) in the hemolymph of infected B. mori incubated for 24 h. Further, Fourier Transform InfraRed studies on the hemolymph extracted from the infected B. mori showed a peak at 1550 cm , indicating the presence of α-helical peptides. The peptide fraction was obtained through methanol, acetic acid and water mixture (90:1:9) extraction, followed by peptide purification using Reverse Phase High Performance Liquid Chromatography. The fraction exhibiting antibacterial properties was collected and characterized by Matrix-Assisted Laser Desorption/Ionization-Time of Flight. A linear α-helical peptide with flexible termini (LLKELWTKMKGAGKAVLGKIKGLL) was found, corresponding to a previously described peptide from ant venom and here denominated as Bm-ponericin-L1. The antibacterial activity of Bm-ponericin-L1 was determined against ESKAPE pathogens. Scanning electron microscopy confirmed the membrane disruption potential of Bm-ponericin-L1. Moreover, this peptide also showed promising antibiofilm activity. Finally, cell viability and hemolytic assays revealed that Bm-ponericin-L1 is non-toxic toward primary fibroblasts cell lines and red blood cells, respectively. This study opens up new perspectives toward an alternative approach to overcoming multiple-antibiotic-resistance by means of AMPs through invertebrates' infection with human pathogenic bacteria.
Editor: England: Nature Publishing Group
Idioma: Inglés

Enlaces

View this record in MEDLINE/PubMed

Volver a la lista de resultados

Anterior Resultado 6 Siguiente Ir a la siguiente página

Realización: Logos de Redes Sociales:

Antimicrobial potential of a ponericin-like peptide isolated from Bombyx mori L. hemolymph in response to Pseudomonas aeruginosa infection

England: Nature Publishing Group

Buscando en bases de datos remotas, por favor espere