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Low-level quantification of melamine and cyanuric acid in limited samples of rat serum by UPLC–electrospray tandem mass spectrometry

Jacob, Cristina C. ; Gamboa da Costa, Gonçalo

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-03, Vol.879 (9), p.652-656 [Periódico revisado por pares]

Amsterdam: Elsevier B.V

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Título:
Low-level quantification of melamine and cyanuric acid in limited samples of rat serum by UPLC–electrospray tandem mass spectrometry
Autor: Jacob, Cristina C. ; Gamboa da Costa, Gonçalo
Assuntos: Analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; blood serum ; Chromatography, High Pressure Liquid - methods ; Cyanuric acid ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; ion exchange ; Mass spectrometry ; Medical sciences ; Melamine ; Methodology ; Monitoring ; Optimization ; Pharmacology. Drug treatments ; Rat serum ; Rats ; Rats, Inbred F344 ; Recovery ; Reproducibility of Results ; Sensitivity and Specificity ; Serums ; Solid Phase Extraction ; Spectrometry, Mass, Electrospray Ionization - methods ; tandem mass spectrometry ; Tandem Mass Spectrometry - methods ; Triazines - blood ; ultra-performance liquid chromatography ; UPLC–ESI-MS/MS
É parte de: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-03, Vol.879 (9), p.652-656
Notas: http://dx.doi.org/10.1016/j.jchromb.2011.01.035
ObjectType-Article-2
SourceType-Scholarly Journals-1
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Descrição: This paper reports the development and validation of a methodology for the low-level quantification of melamine and cyanuric acid in limited samples of rat serum. The methodology, based upon ion-exchange solid phase extraction (SPE) and ultra-performance liquid chromatography (UPLC) coupled with electrospray tandem mass spectrometry (MS/MS) in multiple reaction monitoring (MRM) mode, relies on the use of stable isotope-labeled internal standards and requires only 15 μL samples of serum. The method provides a recovery of 80–110% of melamine with a signal suppression of ca. 55%, and a recovery of 50–90% of cyanuric acid with a signal suppression ca. 40–60%, affording lower limits of quantification (LLOQ) for melamine or cyanuric acid of, respectively, 5 ppb (mean accuracy 109%; CV = 4.9%) and 10 ppb (mean accuracy 96%; CV = 8.6%). The small sample requirements, excellent sensitivity, accuracy and precision, and high-throughput (5 min of instrument run time) make this methodology optimal for toxicokinetic or exposure assessments studies.
Editor: Amsterdam: Elsevier B.V
Idioma: Inglês

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Low-level quantification of melamine and cyanuric acid in limited samples of rat serum by UPLC–electrospray tandem mass spectrometry

Jacob, Cristina C. ; Gamboa da Costa, Gonçalo

Amsterdam: Elsevier B.V

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