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Differential expression profiles of miRNAs and their putative targets in Schistosoma mansoni during its life cycle

Abreu, Fabiano Cp ; Mota, Ester Alves ; Pereira, Roberta V ; Oliveira, Victor F ; Costa, Marcela P ; Gomes, Matheus de S ; Jannotti-Passos, Liana K ; Borges, William C ; Guerra-Sá, Renata

Memórias do Instituto Oswaldo Cruz, 2021-01, Vol.116, p.e200326-e200326 [Periódico revisado por pares]

Brazil: Instituto Oswaldo Cruz, Ministério da Saúde

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  • Título:
    Differential expression profiles of miRNAs and their putative targets in Schistosoma mansoni during its life cycle
  • Autor: Abreu, Fabiano Cp ; Mota, Ester Alves ; Pereira, Roberta V ; Oliveira, Victor F ; Costa, Marcela P ; Gomes, Matheus de S ; Jannotti-Passos, Liana K ; Borges, William C ; Guerra-Sá, Renata
  • Assuntos: Animals ; differential expression ; Gene Expression Profiling ; Gene Expression Regulation - genetics ; Life Cycle Stages - genetics ; MicroRNAs - genetics ; miRNA ; miRNA target genes ; Original ; PARASITOLOGY ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Signal Transduction ; TROPICAL MEDICINE
  • É parte de: Memórias do Instituto Oswaldo Cruz, 2021-01, Vol.116, p.e200326-e200326
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    FCPA - Visualisation, writing - original draft, investigation and formal analysis; EAM - writing - original draft and formal analysis; RVP, MPC and MSG - methodology; VFO - writing - original draft and methodology; LKJ-P and WCB - resources; RG-S - funding acquisition, project administration, supervision, conceptualization and resources. The authors declare that no conflicts of interest.
  • Descrição: Schistosomiasis is a disease caused by Schistosoma. Due to its complex life cycle, evolutionary position and sexual dimorphism, schistosomes have several mechanisms of gene regulation. MicroRNAs (miRNAs) are short endogenous RNAs that regulate gene expression at the post-transcriptional level by targeting mRNA transcripts. Here, we tested 12 miRNAs and identified their putative targets using a computational approach. We performed the expression profiles of a set of miRNAs and their putative targets during the parasite's life cycle by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Our results showed differential expression patterns of the mature miRNAs sma-miR-250; sma-miR-92a; sma-miR-new_4-3p; sma-miR-new_4-5p; sma-miR-new_5-5p; sma-miR-new_12-5p; sma-miR-new_13-3p and sma-miR-new_13-5p. Interestingly, many of the putative target genes are linked to oxidative phosphorylation and are up-regulated in adult-worms, which led us to suggest that miRNAs might play important roles in the post-transcriptional regulation of genes related to energetic metabolism inversion during parasite development. It is noteworthy that the expression of sma-miR-new_13-3p exhibited a negative correlation on SmNADH:ubiquinone oxidoreductase complex I. Our analysis revealed putative miRNA genes related to important biological processes, such as transforming growth factor beta (TGF-β) signaling, proteasome regulation, glucose and lipid metabolism, immune system evasion and transcriptional regulation.
  • Editor: Brazil: Instituto Oswaldo Cruz, Ministério da Saúde
  • Idioma: Inglês;Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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