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In vitro singlet state and zero-quantum encoded magnetic resonance spectroscopy: Illustration with N-acetyl-aspartate

Pravdivtsev, Andrey N ; Sönnichsen, Frank D ; Hövener, Jan-Bernd van der Wel, Patrick

PloS one, 2020-10, Vol.15 (10), p.e0239982-e0239982 [Periódico revisado por pares]

San Francisco: Public Library of Science

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Título:
In vitro singlet state and zero-quantum encoded magnetic resonance spectroscopy: Illustration with N-acetyl-aspartate
Autor: Pravdivtsev, Andrey N ; Sönnichsen, Frank D ; Hövener, Jan-Bernd
van der Wel, Patrick
Assuntos: Aspartate ; Aspartic acid ; Biochemistry ; Biology and Life Sciences ; Brain research ; Chemical equilibrium ; Coupling (molecular) ; Engineering and Technology ; Lipids ; Magnetic properties ; Magnetic resonance ; Magnetic resonance spectroscopy ; Magnetization ; Medicine and Health Sciences ; Metabolism ; Metabolites ; N-Acetyl-L-aspartic acid ; Nuclear magnetic resonance spectroscopy ; Particle spin ; Physical Sciences ; Physics research ; Polarization (spin alignment) ; Proteins ; Protons ; Research and Analysis Methods ; Resonance ; Spectra ; Spectroscopy ; Success
É parte de: PloS one, 2020-10, Vol.15 (10), p.e0239982-e0239982
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Magnetic resonance spectroscopy (MRS) allows the analysis of biochemical processes non-invasively and in vivo. Still, its application in clinical diagnostics is rare. Routine MRS is limited to spatial, chemical and temporal resolutions of cubic centimetres, mM and minutes. In fact, the signal of many metabolites is strong enough for detection, but the resonances significantly overlap, exacerbating identification and quantification. Besides, the signals of water and lipids are much stronger and dominate the entire spectrum. To suppress the background and isolate selected signals, usually, relaxation times, J-coupling and chemical shifts are used. Here, we propose methods to isolate the signals of selected molecular groups within endogenous metabolites by using long-lived spin states (LLS). We exemplify the method by preparing the LLSs of coupled protons in the endogenous molecules N-acetyl-L-aspartic acid (NAA). First, we store polarization in long-lived, double spin states, followed by saturation pulses before the spin order is converted back to observable magnetization or double quantum filters to suppress background signals. We show that LLS and zero-quantum coherences can be used to selectively prepare and measure the signals of chosen metabolites or drugs in the presence of water, inhomogeneous field and highly concentrated fatty solutions. The strong suppression of unwanted signals achieved allowed us to measure pH as a function of chemical shift difference.
Editor: San Francisco: Public Library of Science
Idioma: Inglês

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In vitro singlet state and zero-quantum encoded magnetic resonance spectroscopy: Illustration with N-acetyl-aspartate

Pravdivtsev, Andrey N ; Sönnichsen, Frank D ; Hövener, Jan-Bernd van der Wel, Patrick

San Francisco: Public Library of Science

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