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Synaptic scaffolding proteins in rat brain. Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin

Böckers, T M ; Mameza, M G ; Kreutz, M R ; Bockmann, J ; Weise, C ; Buck, F ; Richter, D ; Gundelfinger, E D ; Kreienkamp, H J

The Journal of biological chemistry, 2001-10, Vol.276 (43), p.40104-40112 [Periódico revisado por pares]

United States

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Título:
Synaptic scaffolding proteins in rat brain. Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin
Autor: Böckers, T M ; Mameza, M G ; Kreutz, M R ; Bockmann, J ; Weise, C ; Buck, F ; Richter, D ; Gundelfinger, E D ; Kreienkamp, H J
Assuntos: a-Fodrin ; Adaptor Proteins, Signal Transducing ; Amino Acid Sequence ; Animals ; Ankyrin Repeat ; Binding Sites ; Brain - embryology ; Brain - ultrastructure ; Brain Chemistry ; Carrier Proteins - isolation & purification ; Carrier Proteins - metabolism ; Conserved Sequence ; Hippocampus - ultrastructure ; Microfilament Proteins - isolation & purification ; Microfilament Proteins - metabolism ; Nerve Tissue Proteins - isolation & purification ; Nerve Tissue Proteins - metabolism ; Protein Binding ; Rats ; Shank protein ; src Homology Domains ; Synapses - chemistry ; Synapses - ultrastructure ; Tissue Distribution ; Two-Hybrid System Techniques
É parte de: The Journal of biological chemistry, 2001-10, Vol.276 (43), p.40104-40112
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
Descrição: The postsynaptic density is the ultrastructural entity containing the neurotransmitter reception apparatus of excitatory synapses in the brain. A recently identified family of multidomain proteins termed Src homology 3 domain and ankyrin repeat-containing (Shank), also known as proline-rich synapse-associated protein/somatostatin receptor-interacting protein, plays a central role in organizing the subsynaptic scaffold by interacting with several synaptic proteins including the glutamate receptors. We used the N-terminal ankyrin repeats of Shank1 and -3 to search for interacting proteins by yeast two-hybrid screening and by affinity chromatography. By cDNA sequencing and mass spectrometry the cytoskeletal protein alpha-fodrin was identified as an interacting molecule. The interaction was verified by pull-down assays and by coimmunoprecipitation experiments from transfected cells and brain extracts. Mapping of the interacting domains of alpha-fodrin revealed that the highly conserved spectrin repeat 21 is sufficient to bind to the ankyrin repeats. Both interacting partners are coexpressed widely in the rat brain and are colocalized in synapses of hippocampal cultures. Our data indicate that the Shank1 and -3 family members provide multiple independent connections between synaptic glutamate receptor complexes and the cytoskeleton.
Editor: United States
Idioma: Inglês

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Synaptic scaffolding proteins in rat brain. Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin

Böckers, T M ; Mameza, M G ; Kreutz, M R ; Bockmann, J ; Weise, C ; Buck, F ; Richter, D ; Gundelfinger, E D ; Kreienkamp, H J

United States

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