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Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624

Sugiura, T.(Shizuoka Univ. (Japan)) ; Yamagishi, K ; Kimura, T ; Nishida, T ; Kawagishi, H ; Hirai, H

Bioscience, biotechnology, and biochemistry, 2009-08, Vol.73 (8), p.1793-1798 [Periódico revisado por pares]

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Título:
Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624
Autor: Sugiura, T.(Shizuoka Univ. (Japan)) ; Yamagishi, K ; Kimura, T ; Nishida, T ; Kawagishi, H ; Hirai, H
Assuntos: AMINO ACID SEQUENCES ; Biological and medical sciences ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; DNA, Complementary - genetics ; DNA, Fungal ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; GENETIC TRANSFORMATION ; Genome, Fungal - genetics ; homologous expression ; LIGNINAS ; LIGNINE ; LIGNINS ; Molecular Sequence Data ; novel lignin peroxidase ; PEROXIDASAS ; PEROXIDASES ; Peroxidases - biosynthesis ; Peroxidases - genetics ; PEROXYDASE ; PHANEROCHAETE ; Phanerochaete - genetics ; Phanerochaete sordida YK-624 ; Plasmids - genetics ; Polymerase Chain Reaction ; PROTEINAS RECOMBINANTES ; PROTEINE RECOMBINANTE ; RECOMBINANT PROTEINS ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; SECUENCIAS DE AMINOACIDOS ; SEQUENCE D'ACIDES AMINES ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Transformation, Genetic
É parte de: Bioscience, biotechnology, and biochemistry, 2009-08, Vol.73 (8), p.1793-1798
Notas: 2009006417
F30
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.
Editor: Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Idioma: Inglês

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Cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus Phanerochaete sordida YK-624

Sugiura, T.(Shizuoka Univ. (Japan)) ; Yamagishi, K ; Kimura, T ; Nishida, T ; Kawagishi, H ; Hirai, H

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

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