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Gene expression profiling of selerenewing stem cells from human umbilical cord blood effects of estradiol

Ana Carolina S. R. Carvalho Luciana C Marti; Patricia R Tobo; Carlos Alberto Moreira-Filho; Oswaldo Keith Okamoto; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)

Abstracts Campinas, SP

Campinas 2004

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  • Título:
    Gene expression profiling of selerenewing stem cells from human umbilical cord blood effects of estradiol
  • Autor: Ana Carolina S. R. Carvalho
  • Luciana C Marti; Patricia R Tobo; Carlos Alberto Moreira-Filho; Oswaldo Keith Okamoto; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)
  • Assuntos: IMUNOLOGIA
  • É parte de: Abstracts Campinas, SP
  • Notas Locais: Disponível em CD-ROM
  • Descrição: Umbilical cord blood (UCB) is an alternative source of stem cells currently employed for hematopoietic reconstitution. However, the small amount of hematopoietic stem cells (HSC) available limits transplantation into adults. In this study, the effects of 17-beta-estradiol (E2) on self-renewal of purified CD34+/CD133+ cells were investigated for ex vivo expansion purpose. Total functional HSC increased 14-times after four weeks-cultivation in stroma-free media containing Flt-3L, SCF, IL-6, and IL-3, as indicated by clonogenic assays. Treatment with 10nM E2 further stimulated self-renewal by a factor of 2.5 and did not alter significantly the proportion of erythroid, granulocytic, and megakaryocytic progenitors. DNAmicroarray analysis of 20,000 human genes revealed 388 differentially expressed genes (?3 fold) after E2-treatment (176 up- and 212 down-regulated), classified in 14 major functional categories (27% of unknown function or without geneontology classification). Microarray data was validated by real-time RTPCR of selected genes, such as hTERT, TKS, TRF1, and POT, involved in telomere maintenance. Our data indicates that E2 is effective in supporting self-renewal of HSC in vitro while keeping to some extent their undifferentiated state. Of particular interest was the activation of genes participating in cell cycle, metabolism and mitochondrial transport, in association with repression of genes regulating telomere degradation and apoptosis
  • Editor: Campinas
  • Data de criação/publicação: 2004
  • Formato: [s. p.].
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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