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Fungal glucuronoyl esterases and substrate uronic acid recognition

Duranova, M., Slovak Academy of Sciences, Bratislava (Slovak R.) ; Hirsch, J ; Kolenova, K ; Biely, P

Bioscience, biotechnology, and biochemistry, 2009-11, Vol.73 (11), p.2483-2487 [Periódico revisado por pares]

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Título:
Fungal glucuronoyl esterases and substrate uronic acid recognition
Autor: Duranova, M., Slovak Academy of Sciences, Bratislava (Slovak R.) ; Hirsch, J ; Kolenova, K ; Biely, P
Assuntos: 4-O-methyl-D-glucuronic acid ; ACTIVIDAD CATALITICA ; ACTIVITE CATALYTIQUE ; Biological and medical sciences ; CATALYTIC ACTIVITY ; CELL WALLS ; ESTERASAS ; ESTERASE ; ESTERASES ; Esterases - chemistry ; Esterases - genetics ; Esterases - isolation & purification ; Esterases - metabolism ; ESTERIFICACION ; ESTERIFICATION ; Fundamental and applied biological sciences. Psychology ; glucuronoxylan ; glucuronoyl esterase ; http://www.fao.org/aos/agrovoc#c_1382 ; http://www.fao.org/aos/agrovoc#c_1415 ; http://www.fao.org/aos/agrovoc#c_2670 ; http://www.fao.org/aos/agrovoc#c_28361 ; http://www.fao.org/aos/agrovoc#c_32212 ; http://www.fao.org/aos/agrovoc#c_36920 ; http://www.fao.org/aos/agrovoc#c_4329 ; Kinetics ; lignin-carbohydrate complex ; LIGNINAS ; LIGNINE ; LIGNINS ; PARED CELULAR ; PAROI CELLULAIRE ; PHANEROCHAETE ; Phanerochaete - enzymology ; Phanerochaete - genetics ; Phanerochaete chrysosporium ; PROTEINAS RECOMBINANTES ; PROTEINE RECOMBINANTE ; RECOMBINANT PROTEINS ; Substrate Specificity ; Uronic Acids - metabolism
É parte de: Bioscience, biotechnology, and biochemistry, 2009-11, Vol.73 (11), p.2483-2487
Notas: F60
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Descrição: Glucuronoyl esterases are enzymes involved in microbial plant cell-wall degradation. In this study we purified and characterized two recombinant Phanerochaete chrysosporium glucuronoyl esterases, PcGE1 and PcGE2. The catalytic activity of these and previously described glucuronoyl esterases was investigated on new synthetic substrates, methyl esters of uronic acids and their glycosides, prepared by esterification with ethereal diazomethane. The data obtained indicate that the enzymes hydrolyzed efficiently not only esters of 4-O-methyl-D-glucuronic acid, but also methyl esters of D-glucuronic acid carrying a 4-nitrophenyl aglycon. Moreover, the fact that they did not recognize the 4-epimers of these compounds, the D-galacturonic acid derivatives, supports the hypothesis that these carbohydrate esterases attack ester linkages between 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols.
Editor: Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Idioma: Inglês

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Fungal glucuronoyl esterases and substrate uronic acid recognition

Duranova, M., Slovak Academy of Sciences, Bratislava (Slovak R.) ; Hirsch, J ; Kolenova, K ; Biely, P

Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry

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