Idioma:

Chemical Exchange at the Trinuclear Copper Center of Small Laccase from Streptomyces coelicolor

Dasgupta, Rubin ; Gupta, Karthick B.S.S. ; Nami, Faezeh ; de Groot, Huub J.M. ; Canters, Gerard W. ; Groenen, Edgar J.J. ; Ubbink, Marcellus

Biophysical journal, 2020-07, Vol.119 (1), p.9-14 [Periódico revisado por pares]

Texto completo disponível

Citações Citado por

Título:
Chemical Exchange at the Trinuclear Copper Center of Small Laccase from Streptomyces coelicolor
Autor: Dasgupta, Rubin ; Gupta, Karthick B.S.S. ; Nami, Faezeh ; de Groot, Huub J.M. ; Canters, Gerard W. ; Groenen, Edgar J.J. ; Ubbink, Marcellus
Assuntos: Biophysical Letter ; Copper ; Electron Spin Resonance Spectroscopy ; Laccase ; Magnetic Resonance Spectroscopy ; Streptomyces coelicolor
É parte de: Biophysical journal, 2020-07, Vol.119 (1), p.9-14
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The trinuclear copper center (TNC) of laccase reduces oxygen to water with very little overpotential. The arrangement of the coppers and ligands in the TNC is known to be from many crystal structures, yet information about possible dynamics of the ligands is absent. Here, we report dynamics at the TNC of small laccase from Streptomyces coelicolor using paramagnetic NMR and electron paramagnetic resonance spectroscopy. Fermi contact-shifted resonances tentatively assigned to histidine Hδ1 display a two-state chemical exchange with exchange rates in the order of 100 s−1. In the electron paramagnetic resonance spectra, at least two forms are observed with different gz-values. It is proposed that the exchange processes reflect the rotational motion of histidine imidazole rings that coordinate the coppers in the TNC. [Display omitted]
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

Voltar para lista de resultados

Anterior Resultado 4 Avançar Ir para próxima página