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Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae

Kallas, Pawel ; Haugen, Håvard J ; Gadegaard, Nikolaj ; Stormonth-Darling, John ; Hulander, Mats ; Andersson, Martin ; Valen, Håkon

Nanomaterials (Basel, Switzerland), 2020-11, Vol.10 (11), p.2247 [Periódico revisado por pares]

Switzerland: MDPI

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  • Título:
    Adhesion of Escherichia Coli to Nanostructured Surfaces and the Role of Type 1 Fimbriae
  • Autor: Kallas, Pawel ; Haugen, Håvard J ; Gadegaard, Nikolaj ; Stormonth-Darling, John ; Hulander, Mats ; Andersson, Martin ; Valen, Håkon
  • Assuntos: anti-adhesive ; anti-bacterial ; biomaterial-associated infections (BAI) ; E. coli ; fimbriae ; injection molding ; nanostructured surface
  • É parte de: Nanomaterials (Basel, Switzerland), 2020-11, Vol.10 (11), p.2247
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the and Δ (with a knockout of major pilus protein FimA) and Δ (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the , no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.
  • Editor: Switzerland: MDPI
  • Idioma: Inglês;Norueguês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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