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The role oligopeptide permease (OPPA) transport system and polyamine content on the resistence to aminoglycosides in Escherichia coli K12

M. B. R Acosta A. C. M Monteiro; Rita de Cássia Café Ferreira; Luís Carlos de Souza Ferreira; S. O. P Costa; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2001

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  • Título:
    The role oligopeptide permease (OPPA) transport system and polyamine content on the resistence to aminoglycosides in Escherichia coli K12
  • Autor: M. B. R Acosta
  • A. C. M Monteiro; Rita de Cássia Café Ferreira; Luís Carlos de Souza Ferreira; S. O. P Costa; Congresso do Instituto de Ciências Biomédicas (3. 2001 São Paulo)
  • Assuntos: MICROBIOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2001
  • Descrição: The oligopeptide permease (Opp) system of Escherichia coli K12 promotes uptake of peptides derived from growth media or recycling of cell wall components. The Opp uptake system has a periplasmic binding element known as OppA which seems to be also involved in cell sensing, pathogenicity and resistance to some antibiotics. Polyamines, particularly putrescine and spermidine, has been shown to post-transcriptionally regulate OppA expression and are essential components of the bacterial metabolism. In this work we evaluated the role of OppA and polyamine expression among in vitro selected aminoglycoside resistant mutants of E. coli J53 and clinically derived E. coli strains. Above 90% of the kanamycin resistant mutants selected at 2 x MIC were defective in OppA expression and showed cross resistance to other aminoglycosides but, in average, no significant reduction in polyamine pools was detected. On the other hand, mutants selected at higher antibiotic concentrations were enriched in mutants affected in respiratory chain or ribosomal components. Resistance to aminoglycoside in OppA-defective mutants was reverted in complementation tests carried out with OppA encoding plasmids. Moreover, OppA deficiency seems to be favorably selected among aminoglycoside E. coli strains isolated from clinical settings. Nine (36%) of 25 aminoglycoside-resistant isolates expressed reduced or undetectable levels of OppA while none was found among aminoglycoside-sensitive strains.
    These results demonstrate the heterogenous nature of spontaneous mutations leading to aminoglycoside resistance and suggest a relevant role of the Opp system on the emergence of antibiotic resistance among laboratory and clinical E. coli strains
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2001
  • Formato: 1 v., pôster 231.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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