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Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Balabushevitch, Nadejda G. ; Sukhorukov, Gleb B. ; Moroz, Natalia A. ; Volodkin, Dmitry V. ; Larionova, Natalia I. ; Donath, Edwin ; Mohwald, Helmuth

Biotechnology and bioengineering, 2001-11, Vol.76 (3), p.207-213 [Periódico revisado por pares]

New York: John Wiley & Sons, Inc

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Título:
Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release
Autor: Balabushevitch, Nadejda G. ; Sukhorukov, Gleb B. ; Moroz, Natalia A. ; Volodkin, Dmitry V. ; Larionova, Natalia I. ; Donath, Edwin ; Mohwald, Helmuth
Assuntos: Adsorption ; Aggregates ; Capsules - chemistry ; Chymotrypsin - chemistry ; Chymotrypsin - metabolism ; colloid ; Encapsulation ; Hydrogen-Ion Concentration ; Membranes ; microcapsules ; Molecular weight ; Polyelectrolytes ; protein aggregates ; protein encapsulation ; protein release ; Solubility ; Substrate Specificity ; Trypsin Inhibitors - chemistry ; Trypsin Inhibitors - metabolism ; ultrathin organic films
É parte de: Biotechnology and bioengineering, 2001-11, Vol.76 (3), p.207-213
Notas: BMBF - No. 03C0293A1
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Descrição: A novel method of protein encapsulation is proposed. Preformed protein aggregates are covered with polyelectrolyte layers by means of layer‐by‐layer adsorption. The polyelectrolyte membrane prevents protein leakage out of the capsule. Using chymotrypsin as a model enzyme the capsule wall selective permeability was demonstrated for substrates and inhibitors of different molecular weight and solubility. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 76: 207–213, 2001.
Editor: New York: John Wiley & Sons, Inc
Idioma: Inglês;Russo

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Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release

Balabushevitch, Nadejda G. ; Sukhorukov, Gleb B. ; Moroz, Natalia A. ; Volodkin, Dmitry V. ; Larionova, Natalia I. ; Donath, Edwin ; Mohwald, Helmuth

New York: John Wiley & Sons, Inc

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