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C-Series Coelenterazine-Driven Bioluminescence Signature Imaging

Kamiya, Genta ; Kitada, Nobuo ; Furuta, Tadaomi ; Hirano, Takashi ; Maki, Shojiro ; Kim, Sung Bae

International journal of molecular sciences, 2022-10, Vol.23 (21), p.13047 [Periódico revisado por pares]

Basel: MDPI AG

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  • Título:
    C-Series Coelenterazine-Driven Bioluminescence Signature Imaging
  • Autor: Kamiya, Genta ; Kitada, Nobuo ; Furuta, Tadaomi ; Hirano, Takashi ; Maki, Shojiro ; Kim, Sung Bae
  • Assuntos: Bioluminescence ; Chromatography ; coelenterazine ; intensity pattern ; luciferase ; Reagents ; reporter ; signature ; Spectral signatures
  • É parte de: International journal of molecular sciences, 2022-10, Vol.23 (21), p.13047
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The present study introduces a unique BL signature imaging system with novel CTZ analogues named “C-series.” Nine kinds of C-series CTZ analogues were first synthesized, and BL intensity patterns and spectra were then examined according to the marine luciferases. The results show that the four CTZ analogues named C3, C4, C6, and C7, individually or collectively luminesce with completely distinctive BL spectral signatures and intensity patterns according to the luciferases: Renilla luciferase (RLuc), NanoLuc, and artificial luciferase (ALuc). The signatural reporters were multiplexed into a multi-reporter system comprising RLuc8.6-535SG and ALuc16. The usefulness of the signatural reporters was further determined with a multi-probe system that consists of two single-chain probes embedding RLuc8 and ALuc23. This study is a great addition to the study of conventional bioassays with a unique methodology, and for the specification of each signal in a single- or multi-reporter system using unique BL signatures and patterns of reporter luciferases.
  • Editor: Basel: MDPI AG
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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