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Comparison of different cytotoxicity assays for in vitro evaluation of mesoporous silica nanoparticles

Braun, Katharina ; Stürzel, Christina Martina ; Biskupek, Johannes ; Kaiser, Ute ; Kirchhoff, Frank ; Lindén, Mika

Toxicology in vitro, 2018-10, Vol.52, p.214-221 [Periódico revisado por pares]

England: Elsevier Ltd

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  • Título:
    Comparison of different cytotoxicity assays for in vitro evaluation of mesoporous silica nanoparticles
  • Autor: Braun, Katharina ; Stürzel, Christina Martina ; Biskupek, Johannes ; Kaiser, Ute ; Kirchhoff, Frank ; Lindén, Mika
  • Assuntos: Assaying ; ATP ; Cell count ; Colorimetry ; Comparative analysis ; Cytotoxicity ; Cytotoxicity assays ; Diagnostic software ; Diagnostic systems ; Evaluation ; Flow cytometry ; Mesoporous silica nanoparticles ; Microscopy ; Nanoparticles ; Optical microscopy ; Silica ; Silicon dioxide ; Surface functionalization ; Therapeutic applications ; Toxicity
  • É parte de: Toxicology in vitro, 2018-10, Vol.52, p.214-221
  • Descrição: Colorimetric or luminogenic cytotoxicity assays are typically applied for in vitro cytotoxicity evaluations due to their easy handling and low cost. However, the results may be strongly assay-dependent. Furthermore, when applied to nanoparticle toxicity screening, nanoparticle-specific interferences can occur. Therefore, it is important to evaluate the assays for different classes of nanoparticles. Mesoporous silica nanoparticles (MSNs) have emerged as a promising platform for both diagnostic and therapeutic applications but a comparison between the commonly employed colorimetric formazan-dependent MTT and WST-1 and luminescent ATP-dependent cytotoxicity assays is still missing. In this work, we evaluated the applicability of four different in vitro cell viability assays for the cytotoxicity analysis of three differently functionalized mesoporous silica nanoparticles towards TZM-bl indicator cells. The results derived from the colorimetric measurements of cell-viability were compared with results obtained by cell count experiments, flow cytometry, and optical microscopy. The correlation between the viability assay results and the viable cell count was observed to be both assay and particle dependent. The MTT assay generally overestimated the cytotoxicity of the mesoporous silica particles, while the WST-1 assay sometimes clearly underestimated their cytotoxicity and even suggested a viability exceeding 100%. Of the two ATP-based assays, the CellTiterGlo assay gave the best correlation with cell count data, although some particle-dependent effects were observed. In conclusion, ATP-based assays seem most suitable for in vitro cytotoxicity evaluation of MSNs. •4 cytotoxicity assays are compared with cell count and FACS analyses of live-dead stained cells.•ROS influences on the cytotoxicity is evaluated using an H2O2 assay.•ATP-based assays outperform WST-1 and MTT assays in cytotoxicity evaluations of MSNs.•The results are confirmed using a second cell line.•MSNs exhibit a low cytotoxicity regardless of their surface characteristics.
  • Editor: England: Elsevier Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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