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Structural and functional divergence of growth hormone-releasing hormone receptors in early sarcopterygians: lungfish and Xenopus

Tam, Janice K V ; Chow, Billy K C ; Lee, Leo T O Vaudry, Hubert

PloS one, 2013-01, Vol.8 (1), p.e53482-e53482 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Structural and functional divergence of growth hormone-releasing hormone receptors in early sarcopterygians: lungfish and Xenopus
Autor: Tam, Janice K V ; Chow, Billy K C ; Lee, Leo T O
Vaudry, Hubert
Assuntos: Affinity ; Amino acids ; Amphibia ; Amphibians ; Analysis ; Animals ; Biological Evolution ; Biology ; Brain ; Calcium ; Calcium (intracellular) ; Calcium - metabolism ; Chickens - genetics ; Chromosome Mapping ; Chromosomes - chemistry ; Chromosomes - genetics ; Cloning ; Crossopterygians ; Cyclic AMP - metabolism ; Danio rerio ; Divergence ; Endocrinology ; Evolution ; Female ; Fishes - genetics ; Fishes - metabolism ; Gene Expression ; Genetic aspects ; Genomes ; Genomics ; Growth hormone ; Growth hormone-releasing hormone ; Growth Hormone-Releasing Hormone - genetics ; Growth Hormone-Releasing Hormone - metabolism ; Growth hormone-releasing hormone receptors ; Homology ; Ligands ; Male ; Mammals ; Medicine ; Nucleotide sequence ; Organ Specificity ; Organs ; Phylogenetics ; Phylogeny ; Physiological aspects ; Physiology ; Pituitary adenylate cyclase-activating polypeptide ; Polypeptides ; Protein Isoforms - chemistry ; Protein Isoforms - classification ; Protein Isoforms - genetics ; Protein Isoforms - metabolism ; Proteins ; Protopterus dolloi ; Receptors ; Receptors, Neuropeptide - chemistry ; Receptors, Neuropeptide - classification ; Receptors, Neuropeptide - genetics ; Receptors, Neuropeptide - metabolism ; Receptors, Pituitary Hormone-Regulating Hormone - chemistry ; Receptors, Pituitary Hormone-Regulating Hormone - classification ; Receptors, Pituitary Hormone-Regulating Hormone - genetics ; Receptors, Pituitary Hormone-Regulating Hormone - metabolism ; Reproductive organs ; Rodents ; Sarcopterygians ; Signal transduction ; Somatotropin releasing hormone ; Species Specificity ; Structure-function relationships ; Tissue analysis ; Vertebrates ; Xenopus - genetics ; Xenopus - metabolism ; Xenopus laevis ; Zebrafish ; Zebrafish - genetics
É parte de: PloS one, 2013-01, Vol.8 (1), p.e53482-e53482
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Conceived and designed the experiments: JKVT LTOL BKCC. Performed the experiments: JKVT LTOL. Analyzed the data: JKVT LTOL. Contributed reagents/materials/analysis tools: LTOL BKCC. Wrote the paper: JKVT BKCC LTOL.
Competing Interests: The last author, LTOL, is a PLOS ONE Editorial Board member. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.
Descrição: The evolutionary trajectories of growth hormone-releasing hormone (GHRH) receptor remain enigmatic since the discovery of physiologically functional GHRH-GHRH receptor (GHRHR) in non-mammalian vertebrates in 2007. Interestingly, subsequent studies have described the identification of a GHRHR(2) in chicken in addition to the GHRHR and the closely related paralogous receptor, PACAP-related peptide (PRP) receptor (PRPR). In this article, we provide information, for the first time, on the GHRHR in sarcopterygian fish and amphibians by the cloning and characterization of GHRHRs from lungfish (P. dolloi) and X. laevis. Sequence alignment and phylogenetic analyses demonstrated structural resemblance of lungfish GHRHR to their mammalian orthologs, while the X. laevis GHRHR showed the highest homology to GHRHR(2) in zebrafish and chicken. Functionally, lungfish GHRHR displayed high affinity towards GHRH in triggering intracellular cAMP and calcium accumulation, while X. laevis GHRHR(2) was able to react with both endogenous GHRH and PRP. Tissue distribution analyses showed that both lungfish GHRHR and X. laevis GHRHR(2) had the highest expression in brain, and interestingly, X. laevis(GHRHR2) also had high abundance in the reproductive organs. These findings, together with previous reports, suggest that early in the Sarcopterygii lineage, GHRHR and PRPR have already established diverged and specific affinities towards their cognate ligands. GHRHR(2), which has only been found in xenopus, zebrafish and chicken hitherto, accommodates both GHRH and PRP.
Editor: United States: Public Library of Science
Idioma: Inglês

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Structural and functional divergence of growth hormone-releasing hormone receptors in early sarcopterygians: lungfish and Xenopus

Tam, Janice K V ; Chow, Billy K C ; Lee, Leo T O Vaudry, Hubert

United States: Public Library of Science

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