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Exploring the potential of novel Bacillus sp. G6: Isolation, characterization, and optimization of biosurfactant production from oil-contaminated soil

Rawat, Geeta ; Kumar, Vijay ; Kumar, Akhilesh ; Naik, Bindu ; Saris, Per Erik Joakim ; Khan, Javed Masood ; Kumar, Vivek

Journal of molecular liquids, 2024-02, Vol.396, p.124013, Article 124013 [Periódico revisado por pares]

Elsevier B.V

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  • Título:
    Exploring the potential of novel Bacillus sp. G6: Isolation, characterization, and optimization of biosurfactant production from oil-contaminated soil
  • Autor: Rawat, Geeta ; Kumar, Vijay ; Kumar, Akhilesh ; Naik, Bindu ; Saris, Per Erik Joakim ; Khan, Javed Masood ; Kumar, Vivek
  • Assuntos: Bacillus ; Biosurfactants ; Molasses ; Oil-contaminated soils ; Optimization ; Response surface methodology
  • É parte de: Journal of molecular liquids, 2024-02, Vol.396, p.124013, Article 124013
  • Descrição: •Statistical optimization of BS production using PBD and Box-Behnken design by novel Bacillus species.•Highest BS yield of 3.79 g/L was produced with 1.92-fold increase in yield.•RSM and Box-Behnken design optimized factors are Na2HPO4, FeSO4, and waste oil.•Biosurfactant from Bacillus sp. G6 identified as surfactin, exhibited stability in diverse conditions. Biosurfactants (BSs) are highly biodegradable, environmentally benign, and multifunctional substances having diverse industrial uses, including environment bioremediation. Because of its remarkable emulsifying properties, as well as its use in both the environment and medicine, surfactin, is considered one of the most potent BSs and belongs to the lipopeptides family. The present study was performed to isolate the potential bacteria from oil-contaminated soils. Selected bacteria were checked for biosurfactant (BS) synthesis and their characterization. Out of 32 bacteria, the most promising isolate G6 was taken for a detailed study. Based on polyphasic taxonomy it was found to belong to the novelBacillussp.Nutrient screening for higher BS synthesis usingthe Plackett-Burman design(PBD) showed thatFeSO4, NaCl andwaste engineoil have a significant influence on BS production. Further optimization utilizingBox-Behnken design(BBD) revealed that the optimized condition for higher BS synthesis wasFeSO4(2.0 %), NaCl (2.0 %), and waste engine oil (2.2 %). The highest BS yield of 3.79 ± 0.265 g/L was attained with a cell biomass of 1.51 ± 0.234 g/L with 1.92 fold increase in the yield. Based on thin-layer chromatogram (TLC),Fourier transform infrared spectroscopy(FTIR), andGas Chromatogram-Mass Spectrometry(GC–MS) studies it was observed that the probable BS was a surfactin-like molecule that is stable at a wide range of temperature (up to 100° C), pH (5–9), and salinity (3–6 %).
  • Editor: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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