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A streamlined method for the fast and cost-effective detection of bacterial pathogens from positive blood cultures for the BacT/ALERT blood culture system using the Vitek MS mass spectrometer

Forster, Johannes ; Kohlmorgen, Britta ; Haas, Julian ; Weis, Philipp ; Breunig, Lukas ; Turnwald, Doris ; Mizaikoff, Boris ; Schoen, Christoph Banoub, Joseph

PloS one, 2022-04, Vol.17 (4), p.e0267669-e0267669 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A streamlined method for the fast and cost-effective detection of bacterial pathogens from positive blood cultures for the BacT/ALERT blood culture system using the Vitek MS mass spectrometer
Autor: Forster, Johannes ; Kohlmorgen, Britta ; Haas, Julian ; Weis, Philipp ; Breunig, Lukas ; Turnwald, Doris ; Mizaikoff, Boris ; Schoen, Christoph
Banoub, Joseph
Assuntos: Antibiotics ; Bacteria ; Biology and Life Sciences ; Blood ; Blood Culture ; Bottles ; Centrifugation ; Cost-Benefit Analysis ; Engineering and Technology ; Gram-negative bacteria ; Gram-positive bacteria ; Health aspects ; Hygiene ; Internal medicine ; Laboratories ; Lysis ; Mass spectrometry ; Medicine and Health Sciences ; Methods ; Pathogenic microorganisms ; Pathogens ; Prospective Studies ; Sample preparation ; Specimen Handling - methods ; Spectrometer
É parte de: PloS one, 2022-04, Vol.17 (4), p.e0267669-e0267669
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Prompt pathogen identification of blood stream infections is essential to provide appropriate antibiotic treatment. Therefore, the objective of this prospective single centre study was to establish an inexpensive, fast and accurate protocol for bacterial species identification with SDS protein-extraction directly from BacT/Alert® blood culture (BC) bottles by VitekMS®. Correct species identification was obtained for 198/266 (74.4%, 95%-CI = [68.8%, 79.6%]) of pathogens. The protocol was more successful in identifying 87/96 (91.4%, 95%-CI = [83.8%, 93.2%]) gram-negative bacteria than 110/167 (65.9%, 95%-CI = [58.1%, 73.0%]) gram-positive bacteria. The hands-on time for sample preparation and measurement was about 15 min for up to five samples. This is shorter than for most other protocols using a similar lysis-centrifugation approach for the combination of BacT/Alert® BC bottles and the Vitek® MS mass spectrometer. The estimated costs per sample were approx. 1.80€ which is much cheaper than for commercial kits. This optimized protocol allows for accurate identification of bacteria directly from blood culture bottles for laboratories equipped with BacT/Alert® blood culture bottles and VitekMS® mass spectrometer.
Editor: United States: Public Library of Science
Idioma: Inglês

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A streamlined method for the fast and cost-effective detection of bacterial pathogens from positive blood cultures for the BacT/ALERT blood culture system using the Vitek MS mass spectrometer

Forster, Johannes ; Kohlmorgen, Britta ; Haas, Julian ; Weis, Philipp ; Breunig, Lukas ; Turnwald, Doris ; Mizaikoff, Boris ; Schoen, Christoph Banoub, Joseph

United States: Public Library of Science

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