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Spatial and temporal diversity of DCLK1 isoforms in developing mouse brain

Bergoglio, Emilia ; Suzuki, Ikuo K. ; Togashi, Kazuya ; Tsuji, Masato ; Takeuchi, Shunsuke ; Koizumi, Hiroyuki ; Emoto, Kazuo

Neuroscience research, 2021-09, Vol.170, p.154-165 [Periódico revisado por pares]

Ireland: Elsevier B.V

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  • Título:
    Spatial and temporal diversity of DCLK1 isoforms in developing mouse brain
  • Autor: Bergoglio, Emilia ; Suzuki, Ikuo K. ; Togashi, Kazuya ; Tsuji, Masato ; Takeuchi, Shunsuke ; Koizumi, Hiroyuki ; Emoto, Kazuo
  • Assuntos: Animals ; Brain development ; Cell Movement ; Cerebral Cortex - metabolism ; Dendrites ; Doublecortin family proteins ; Doublecortin-Like Kinases ; Intracellular Signaling Peptides and Proteins - genetics ; Mice ; Neuronal migration ; Protein Isoforms - genetics ; Protein Serine-Threonine Kinases - genetics
  • É parte de: Neuroscience research, 2021-09, Vol.170, p.154-165
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: •In silico analysis of DCLK1 transcripts reveals developmental dynamics unique for each isoform.•DCLK1 isoforms are distributed in the partially distinct brain regions.•Overexpression of DCLK1-L in progenitors causes neural migration defects.•The migration defects by DCLK1-L overexpression requires its kinase activity Doublecortin-like kinase 1 (DCLK1) is a Doublecortin family kinase involved in a range of brain development processes including cell migration, axon/dendrite growth, and synapse development. The Dclk1 gene potentially generates multiple splicing isoforms, but the detailed expression patterns in the brain as well as in vivo functions of each isoform are still incompletely understood. Here we assessed expression patterns of DCLK1 isoforms using multiple platforms including in silico, in situ, and in vitro datasets in the developing mouse brain, and show quantitative evidence that among the four DCLK1 isoforms, DCLK1-L and DCL are mainly expressed in the embryonic cortex whereas DCLK1-L and CPG16 become dominant compared to DCL and CARP in the postnatal cortex. We also provide compelling evidence that DCLK1 isoforms are distributed in the partially distinct brain regions in the embryonic and the postnatal stages. We further show that overexpression of DCLK1-L, but not the other isoforms, in neural progenitors causes severe migration defects in the cortex, and that the migration defects are dependent on the kinase activity of DCLK1-L. Our data thus uncover partially segregated localization of DCLK1 isoforms in the developing mouse brain and suggest different roles for distinct DCLK1 isoforms in the brain development and function.
  • Editor: Ireland: Elsevier B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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