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Assembly and development of the Pseudomonas aeruginosa biofilm matrix

Ma, Luyan ; Conover, Matthew ; Lu, Haiping ; Parsek, Matthew R ; Bayles, Kenneth ; Wozniak, Daniel J Ausubel, Frederick M.

PLoS pathogens, 2009-03, Vol.5 (3), p.e1000354-e1000354 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Assembly and development of the Pseudomonas aeruginosa biofilm matrix
Autor: Ma, Luyan ; Conover, Matthew ; Lu, Haiping ; Parsek, Matthew R ; Bayles, Kenneth ; Wozniak, Daniel J
Ausubel, Frederick M.
Assuntos: Apoptosis ; Bacteria ; Bacteriology ; Biochemistry/Cell Signaling and Trafficking Structures ; Biofilms ; Biofilms - growth & development ; Cellulase ; Colleges & universities ; Deoxyribonucleic acid ; Developmental Biology/Cell Differentiation ; DNA ; DNA, Bacterial ; Extracellular matrix ; Extracellular Matrix - chemistry ; Extracellular Matrix - metabolism ; Extracellular Matrix - ultrastructure ; Genes, Bacterial ; Genetic aspects ; Health aspects ; Health sciences ; Infections ; Infectious Diseases/Antimicrobials and Drug Resistance ; Infectious Diseases/Bacterial Infections ; Infectious Diseases/Respiratory Infections ; Microbial mats ; Microbiology ; Microbiology/Cellular Microbiology and Pathogenesis ; Microbiology/Medical Microbiology ; Microbiology/Microbial Growth and Development ; Microscopy, Confocal ; Polysaccharides, Bacterial - chemistry ; Polysaccharides, Bacterial - metabolism ; Polysaccharides, Bacterial - ultrastructure ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - physiology
É parte de: PLoS pathogens, 2009-03, Vol.5 (3), p.e1000354-e1000354
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Current address: Division of Infectious Disease, Center for Microbial Interface Biology, The Ohio State University, Columbus, Ohio, United States of America
Conceived and designed the experiments: LM MC MRP KWB DJW. Performed the experiments: LM MC HL. Analyzed the data: LM MC HL MRP KWB DJW. Contributed reagents/materials/analysis tools: KWB. Wrote the paper: LM MRP KWB DJW.
Descrição: Virtually all cells living in multicellular structures such as tissues and organs are encased in an extracellular matrix. One of the most important features of a biofilm is the extracellular polymeric substance that functions as a matrix, holding bacterial cells together. Yet very little is known about how the matrix forms or how matrix components encase bacteria during biofilm development. Pseudomonas aeruginosa forms environmentally and clinically relevant biofilms and is a paradigm organism for the study of biofilms. The extracellular polymeric substance of P. aeruginosa biofilms is an ill-defined mix of polysaccharides, nucleic acids, and proteins. Here, we directly visualize the product of the polysaccharide synthesis locus (Psl exopolysaccharide) at different stages of biofilm development. During attachment, Psl is anchored on the cell surface in a helical pattern. This promotes cell-cell interactions and assembly of a matrix, which holds bacteria in the biofilm and on the surface. Chemical dissociation of Psl from the bacterial surface disrupted the Psl matrix as well as the biofilm structure. During biofilm maturation, Psl accumulates on the periphery of 3-D-structured microcolonies, resulting in a Psl matrix-free cavity in the microcolony center. At the dispersion stage, swimming cells appear in this matrix cavity. Dead cells and extracellular DNA (eDNA) are also concentrated in the Psl matrix-free area. Deletion of genes that control cell death and autolysis affects the formation of the matrix cavity and microcolony dispersion. These data provide a mechanism for how P. aeruginosa builds a matrix and subsequently a cavity to free a portion of cells for seeding dispersal. Direct visualization reveals that Psl is a key scaffolding matrix component and opens up avenues for therapeutics of biofilm-related complications.
Editor: United States: Public Library of Science
Idioma: Inglês

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Assembly and development of the Pseudomonas aeruginosa biofilm matrix

Ma, Luyan ; Conover, Matthew ; Lu, Haiping ; Parsek, Matthew R ; Bayles, Kenneth ; Wozniak, Daniel J Ausubel, Frederick M.

United States: Public Library of Science

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