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Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

Maignan, Maxime ; Viglino, Damien ; Hablot, Maud ; Termoz Masson, Nicolas ; Lebeugle, Anne ; Collomb Muret, Roselyne ; Mabiala Makele, Prudence ; Guglielmetti, Valérie ; Morand, Patrice ; Lupo, Julien ; Forget, Virginie ; Landelle, Caroline ; Larrat, Sylvie Mayr, Florian B.

PloS one, 2019-05, Vol.14 (5), p.e0216308-e0216308 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season
Autor: Maignan, Maxime ; Viglino, Damien ; Hablot, Maud ; Termoz Masson, Nicolas ; Lebeugle, Anne ; Collomb Muret, Roselyne ; Mabiala Makele, Prudence ; Guglielmetti, Valérie ; Morand, Patrice ; Lupo, Julien ; Forget, Virginie ; Landelle, Caroline ; Larrat, Sylvie
Mayr, Florian B.
Assuntos: Accuracy ; Acids ; Adult ; Aged ; Aged, 80 and over ; Assaying ; Biochemistry, Molecular Biology ; Biology and Life Sciences ; Confidence intervals ; Diagnosis ; Diagnostic systems ; Disease control ; Drug resistance ; Emergencies ; Emergency medical services ; Emergency nursing ; Emergency service ; Emergency Service, Hospital ; Emergency services ; Evaluation ; Female ; France - epidemiology ; Hospital emergency services ; Hospitals ; Human health and pathology ; Humans ; Infections ; Infectious diseases ; Influenza ; Influenza A ; Influenza A virus - genetics ; Influenza B virus - genetics ; Influenza, Human - diagnosis ; Influenza, Human - epidemiology ; Influenza, Human - genetics ; Influenza, Human - virology ; Laboratories ; Laboratory diagnosis ; Life Sciences ; Male ; Management ; Medical diagnosis ; Medical personnel ; Medical research ; Medicine and Health Sciences ; Medicine, Experimental ; Methods ; Middle Aged ; Molecular Diagnostic Techniques ; Nurses ; Patients ; People and Places ; Point of care testing ; Point-of-Care Systems ; Polymerase chain reaction ; Prospective Studies ; Research and Analysis Methods ; Reverse Transcriptase Polymerase Chain Reaction ; Seasons ; Specimen Handling ; Triage (Medicine) ; Viruses
É parte de: PloS one, 2019-05, Vol.14 (5), p.e0216308-e0216308
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
PMCID: PMC6504036
Competing Interests: Roche Diagnostics had no role in the study design; collection, analysis, or interpretation of data; writing of the paper; and/or decision to submit for publication. None of the authors received direct funding from this company. MundiPharma and Purdue: These sister companies had no role in this study and are currently working outside the field of influenza diagnosis or treatment. MM and DV received a grant to conduct a trial on pain management in the ED (NCT03380247). MM also received consultant fees as a “pain management expert” from these companies. DV received a grant from AstraZenecca to conducted biological analysis in a subgroup of patients from a larger study investigating exacerbation of Chronic Obstructive Pulmonary Disease (NCT03474575). This company had no role in the present study. DV did not receive fee from this company. These competing interests do not alter our adherence to PLOS ONE policies on sharing data and materials.
Descrição: To investigate the performance of a rapid RT-PCR assay to detect influenza A/B at emergency department admission. This single-center prospective study recruited adult patients attending the emergency department for influenza-like illness. Triage nurses performed nasopharyngeal swab samples and ran rapid RT-PCR assays using a dedicated device (cobas Liat, Roche Diagnostics, Meylan, France) located at triage. The same swab sample was also analyzed in the department of virology using conventional RT-PCR techniques. Patients were included 24 hours-a-day, 7 days-a-week. The primary outcome was the diagnostic accuracy of the rapid RT-PCR assay performed at triage. A total of 187 patients were included over 11 days in January 2018. Median age was 70 years (interquartile range 44 to 84) and 95 (51%) were male. Nine (5%) assays had to be repeated due to failure of the first assay. The sensitivity of the rapid RT-PCR assay performed at triage was 0.98 (95% confidence interval (CI): 0.91-1.00) and the specificity was 0.99 (95% CI: 0.94-1.00). A total of 92 (49%) assays were performed at night-time or during the weekend. The median time from patient entry to rapid RT-PCR assay results was 46 [interquartile range 36-55] minutes. Rapid RT-PCR assay performed by nurses at triage to detect influenza A/B is feasible and highly accurate.
Editor: United States: Public Library of Science
Idioma: Inglês

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Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season

Maignan, Maxime ; Viglino, Damien ; Hablot, Maud ; Termoz Masson, Nicolas ; Lebeugle, Anne ; Collomb Muret, Roselyne ; Mabiala Makele, Prudence ; Guglielmetti, Valérie ; Morand, Patrice ; Lupo, Julien ; Forget, Virginie ; Landelle, Caroline ; Larrat, Sylvie Mayr, Florian B.

United States: Public Library of Science

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