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Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis

Zhang, Hua ; Zou, Pan ; Zhao, Haitian ; Qiu, Junqiang ; Regenstein, Joe Mac ; Yang, Xin

Carbohydrate polymers, 2021-01, Vol.251, p.117078, Article 117078 [Periódico revisado por pares]

England: Elsevier Ltd

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Título:
Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis
Autor: Zhang, Hua ; Zou, Pan ; Zhao, Haitian ; Qiu, Junqiang ; Regenstein, Joe Mac ; Yang, Xin
Assuntos: Antioxidants - chemistry ; Antioxidants - pharmacology ; Arabinose - chemistry ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - pharmacology ; Functional Food ; Galactose - chemistry ; Glucose - chemistry ; Hydroxyl Radical - chemistry ; Mannose - chemistry ; Monosaccharides - chemistry ; Oxidation-Reduction ; Pinecones ; Pinus - chemistry ; Pinus koraiensis ; PKP-E ; Polysaccharide ; Polysaccharides - chemistry ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; Rhamnose - chemistry ; Structure characterization
É parte de: Carbohydrate polymers, 2021-01, Vol.251, p.117078, Article 117078
Descrição: •Four novel homogeneous polysaccharides with antioxidant activities were isolated from PKP-E.•The structural features of the 4 fractions were characterized using multiple analysis methods.•The backbone of 4 fractions contained →2, 6)-β-d-Man-(1→, and →6)-α-d-GalpA-(1→), analogous to pectin.•PKP-E-2-1 was the most stable and active fraction by hydroxyl and ABTS+ scavenging assays. The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1−1, -1−2, -2−1, and -2−2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2−1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.
Editor: England: Elsevier Ltd
Idioma: Inglês

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Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis

Zhang, Hua ; Zou, Pan ; Zhao, Haitian ; Qiu, Junqiang ; Regenstein, Joe Mac ; Yang, Xin

England: Elsevier Ltd

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