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Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions

Hoernes, Thomas Philipp ; Faserl, Klaus ; Juen, Michael Andreas ; Kremser, Johannes ; Gasser, Catherina ; Fuchs, Elisabeth ; Shi, Xinying ; Siewert, Aaron ; Lindner, Herbert ; Kreutz, Christoph ; Micura, Ronald ; Joseph, Simpson ; Höbartner, Claudia ; Westhof, Eric ; Hüttenhofer, Alexander ; Erlacher, Matthias David

Nature communications, 2018-11, Vol.9 (1), p.4865-12, Article 4865 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions
Autor: Hoernes, Thomas Philipp ; Faserl, Klaus ; Juen, Michael Andreas ; Kremser, Johannes ; Gasser, Catherina ; Fuchs, Elisabeth ; Shi, Xinying ; Siewert, Aaron ; Lindner, Herbert ; Kreutz, Christoph ; Micura, Ronald ; Joseph, Simpson ; Höbartner, Claudia ; Westhof, Eric ; Hüttenhofer, Alexander ; Erlacher, Matthias David
Assuntos: 2-Aminopurine - analogs & derivatives ; 2-Aminopurine - chemistry ; 2-Aminopurine - metabolism ; Anticodon - chemistry ; Anticodon - metabolism ; Bacteriophage T7 - genetics ; Bacteriophage T7 - metabolism ; Base Sequence ; Biochemistry ; Biochemistry, Molecular Biology ; Codon - chemistry ; Codon - metabolism ; Cytidine - analogs & derivatives ; Cytidine - genetics ; Cytidine - metabolism ; Decoding ; Destabilization ; DNA-Directed RNA Polymerases - genetics ; DNA-Directed RNA Polymerases - metabolism ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Geometry ; HEK293 Cells ; Humans ; Hydrogen Bonding ; Hydrogen bonds ; Inosine - genetics ; Inosine - metabolism ; Life Sciences ; Molecular biology ; mRNA ; Nucleotides ; Organic chemistry ; Protein Biosynthesis ; Protein synthesis ; Proteins ; Purines ; Pyridones - chemistry ; Pyridones - metabolism ; Pyrimidines ; Receptor, Serotonin, 5-HT2C - genetics ; Receptor, Serotonin, 5-HT2C - metabolism ; Ribonucleic acid ; Ribosomes ; Ribosomes - genetics ; Ribosomes - metabolism ; RNA ; RNA, Transfer, Gly - genetics ; RNA, Transfer, Gly - metabolism ; Serotonin ; Serotonin S2 receptors ; Translation ; tRNA ; Viral Proteins - genetics ; Viral Proteins - metabolism
É parte de: Nature communications, 2018-11, Vol.9 (1), p.4865-12, Article 4865
Notas: PMCID: PMC6242847
Descrição: The precise interplay between the mRNA codon and the tRNA anticodon is crucial for ensuring efficient and accurate translation by the ribosome. The insertion of RNA nucleobase derivatives in the mRNA allowed us to modulate the stability of the codon-anticodon interaction in the decoding site of bacterial and eukaryotic ribosomes, allowing an in-depth analysis of codon recognition. We found the hydrogen bond between the N of purines and the N of pyrimidines to be sufficient for decoding of the first two codon nucleotides, whereas adequate stacking between the RNA bases is critical at the wobble position. Inosine, found in eukaryotic mRNAs, is an important example of destabilization of the codon-anticodon interaction. Whereas single inosines are efficiently translated, multiple inosines, e.g., in the serotonin receptor 5-HT mRNA, inhibit translation. Thus, our results indicate that despite the robustness of the decoding process, its tolerance toward the weakening of codon-anticodon interactions is limited.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Translation of non-standard codon nucleotides reveals minimal requirements for codon-anticodon interactions

England: Nature Publishing Group

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