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Establishing zebrafish as a novel exercise model: swimming economy, swimming-enhanced growth and muscle growth marker gene expression

Palstra, Arjan P ; Tudorache, Christian ; Rovira, Mireia ; Brittijn, Sebastiaan A ; Burgerhout, Erik ; van den Thillart, Guido E E J M ; Spaink, Herman P ; Planas, Josep V Calbet, Jose A. L.

PloS one, 2010-12, Vol.5 (12), p.e14483-e14483 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Establishing zebrafish as a novel exercise model: swimming economy, swimming-enhanced growth and muscle growth marker gene expression
Autor: Palstra, Arjan P ; Tudorache, Christian ; Rovira, Mireia ; Brittijn, Sebastiaan A ; Burgerhout, Erik ; van den Thillart, Guido E E J M ; Spaink, Herman P ; Planas, Josep V
Calbet, Jose A. L.
Assuntos: Analysis ; Animal models in research ; Animals ; Aquaculture ; Base Sequence ; Biology ; Body length ; Body weight ; Calcium-binding protein ; Cell division ; Consumption ; Creixement ; Danio rerio ; DNA Primers - genetics ; Economic aspects ; Energy Metabolism - genetics ; Exercise ; Exercise physiology ; Experiments ; Fish ; Fishes ; Gene expression ; Genes ; Growth ; Growth hormone ; Infections ; Insulin ; Insulin-like growth factors ; Laboratories ; Metabolism ; Models animals en la investigació ; Models, Animal ; Models, Biological ; Molecular Sequence Data ; Muscles ; Muscles - metabolism ; Musculoskeletal system ; Myosin ; Myostatin ; Músculs ; Oncorhynchus mykiss ; Oxygen Consumption ; Peix zebra ; Physical Conditioning, Animal ; Physical fitness ; Physical training ; Physiological aspects ; Physiology ; Physiology/Muscle and Connective Tissue ; Physiology/Respiratory Physiology ; Polymerase Chain Reaction - methods ; Salmonidae ; Somatotropin ; Studies ; Swimming ; Swimming - physiology ; Transport ; Troponin ; Troponin C ; Trout ; Zebra danio ; Zebrafish
É parte de: PloS one, 2010-12, Vol.5 (12), p.e14483-e14483
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Conceived and designed the experiments: AP CT GvdT HPS JVP. Performed the experiments: AP CT MR SB EB. Analyzed the data: AP CT. Contributed reagents/materials/analysis tools: AP. Wrote the paper: AP CT GvdT JVP.
Descrição: Zebrafish has been largely accepted as a vertebrate multidisciplinary model but its usefulness as a model for exercise physiology has been hampered by the scarce knowledge on its swimming economy, optimal swimming speeds and cost of transport. Therefore, we have performed individual and group-wise swimming experiments to quantify swimming economy and to demonstrate the exercise effects on growth in adult zebrafish. Individual zebrafish (n = 10) were able to swim at a critical swimming speed (U(crit)) of 0.548±0.007 m s(-1) or 18.0 standard body lengths (BL) s(-1). The optimal swimming speed (U(opt)) at which energetic efficiency is highest was 0.396±0.019 m s(-1) (13.0 BL s(-1)) corresponding to 72.26±0.29% of U(crit). The cost of transport at optimal swimming speed (COT(opt)) was 25.23±4.03 µmol g(-1) m(-1). A group-wise experiment was conducted with zebrafish (n = 83) swimming at U(opt) for 6 h day(-1) for 5 days week(-1) for 4 weeks vs. zebrafish (n = 84) that rested during this period. Swimming zebrafish increased their total body length by 5.6% and body weight by 41.1% as compared to resting fish. For the first time, a highly significant exercise-induced growth is demonstrated in adult zebrafish. Expression analysis of a set of muscle growth marker genes revealed clear regulatory roles in relation to swimming-enhanced growth for genes such as growth hormone receptor b (ghrb), insulin-like growth factor 1 receptor a (igf1ra), troponin C (stnnc), slow myosin heavy chain 1 (smyhc1), troponin I2 (tnni2), myosin heavy polypeptide 2 (myhz2) and myostatin (mstnb). From the results of our study we can conclude that zebrafish can be used as an exercise model for enhanced growth, with implications in basic, biomedical and applied sciences, such as aquaculture.
Editor: United States: Public Library of Science
Idioma: Inglês

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Establishing zebrafish as a novel exercise model: swimming economy, swimming-enhanced growth and muscle growth marker gene expression

Palstra, Arjan P ; Tudorache, Christian ; Rovira, Mireia ; Brittijn, Sebastiaan A ; Burgerhout, Erik ; van den Thillart, Guido E E J M ; Spaink, Herman P ; Planas, Josep V Calbet, Jose A. L.

United States: Public Library of Science

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