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Measuring the Refractive Index and Its Two-Dimensional Distribution of Picoliter Microfluidics With the Scanning White Light Interference Microscopy Method

Xia, Mengrou ; Wu, Rongyao ; Zhan, Wei ; Ji, Xiaoyuan ; Guo, Lianbo ; Song, Wuzhou

IEEE access, 2020, Vol.8, p.190553-190558 [Periódico revisado por pares]

Piscataway: IEEE

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Título:
Measuring the Refractive Index and Its Two-Dimensional Distribution of Picoliter Microfluidics With the Scanning White Light Interference Microscopy Method
Autor: Xia, Mengrou ; Wu, Rongyao ; Zhan, Wei ; Ji, Xiaoyuan ; Guo, Lianbo ; Song, Wuzhou
Assuntos: Chemical analysis ; Deionization ; Interference ; Liquid paraffins ; Liquids ; Medical materials ; Medical research ; Microfluidics ; Microscopy ; Optical imaging ; Optical reflection ; Optical refraction ; picoliters ; Refractive index ; refractive index measurement ; Refractivity ; scanning white light interference ; two-dimensional distribution of refractive index ; White light
É parte de: IEEE access, 2020, Vol.8, p.190553-190558
Descrição: Measurements on refractive index and its distribution of microfluidics are widely applied in the field of biological detection, chemical analysis, material researches, medical diagnostics, and lab on a chip. Since the volume of the microfluidics is ultra-small, such as nanoliters or even picoliters, measuring the refractive index of microfluidics is challenging. We here presents an effective method to measure the refractive index and its two-dimensional distribution of picoliter microfluidics by the SWLI (scanning white light interference) microscopy technology. This method has the advantages of simplicity, low cost, non-contact, wide measurement range and high precision. We conduct the experiment to measure the refractive index of 2.4 picoliters liquid. The resolution is 10 −4 RIU (refractive index unit), and the relative error is 10 −4 compared with the Abbe refractometer. We obtained the relationship between refractive index and concentration of glucose solution. In addition, we can measure the 2D (two-dimensional) distribution of RI (refractive index) of liquids. We can observe the liquid interface between deionized water and liquid paraffin clearly. Besides, we can observe the process of solute dissolution in the liquid and the change of refractive index.
Editor: Piscataway: IEEE
Idioma: Inglês

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Measuring the Refractive Index and Its Two-Dimensional Distribution of Picoliter Microfluidics With the Scanning White Light Interference Microscopy Method

Xia, Mengrou ; Wu, Rongyao ; Zhan, Wei ; Ji, Xiaoyuan ; Guo, Lianbo ; Song, Wuzhou

Piscataway: IEEE

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