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Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season

Shrivastava, Shubham ; Tiraki, Divya ; Diwan, Arundhati ; Lalwani, Sanjay K ; Modak, Meera ; Mishra, Akhilesh Chandra ; Arankalle, Vidya A Ansari, Aftab A.

PloS one, 2018-02, Vol.13 (2), p.e0192672-e0192672 [Periódico revisado por pares]

United States: Public Library of Science

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Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season
Autor: Shrivastava, Shubham ; Tiraki, Divya ; Diwan, Arundhati ; Lalwani, Sanjay K ; Modak, Meera ; Mishra, Akhilesh Chandra ; Arankalle, Vidya A
Ansari, Aftab A.
Assuntos: Aquatic insects ; Biology and life sciences ; Cell culture ; Computer and Information Sciences ; Concurrent infection ; Consent ; Dengue ; Dengue fever ; Dengue virus ; Dengue viruses ; Disease ; Enzyme-linked immunosorbent assay ; Gene sequencing ; Genetic aspects ; Genomes ; Genomics ; Genotype & phenotype ; Genotypes ; Genotyping ; Health aspects ; Hospitals ; Infection ; Infections ; Medicine and Health Sciences ; Nucleotide sequence ; Outbreaks ; People and Places ; Phylogenetics ; Phylogeny ; Physical Sciences ; Research and Analysis Methods ; Serotypes ; Vector-borne diseases ; Viral diseases ; Viruses
É parte de: PloS one, 2018-02, Vol.13 (2), p.e0192672-e0192672
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Competing Interests: The authors have declared that no competing interests exist.
Descrição: Dengue is the most common mosquito-borne viral infection in tropical and sub-tropical countries. In recent years, India has reported increased incidences of concurrent infection with multiple serotypes of dengue viruses (DENV). In the present study, we have characterized DENV circulating during a single season of 2016 in Pune, India. A total of 64 serum samples from NS1 ELISA positive dengue patients were used for PCR amplification of CprM region of the viral genome and sequencing. Phylogenetic analysis documented circulation of all the four DENV serotypes with predominance of DENV-2 (40.6%). DENV genotyping classified DENV-1 to Genotype V, DENV-2 to Genotype IV, DENV-3 to Genotype III and DENV-4 to Genotype I. Further analysis revealed emergence of a novel clade (D) of genotype I of DENV-4. Subsequent isolation of three DENV-4 viruses in cell culture followed by complete genome sequence analysis confirmed this observation. Additionally, a new genotype within serotype-4 with >6.7% sequence variation from other genotypes was identified. This first report of significant co-circulation of all the four serotypes in a single outbreak in Pune reconfirms need for molecular monitoring of DENV.
Editor: United States: Public Library of Science
Idioma: Inglês

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Co-circulation of all the four dengue virus serotypes and detection of a novel clade of DENV-4 (genotype I) virus in Pune, India during 2016 season

Shrivastava, Shubham ; Tiraki, Divya ; Diwan, Arundhati ; Lalwani, Sanjay K ; Modak, Meera ; Mishra, Akhilesh Chandra ; Arankalle, Vidya A Ansari, Aftab A.

United States: Public Library of Science

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