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Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines

Debeb, Bisrat G ; Galat, Vasiliy ; Epple-Farmer, Jessica ; Iannaccone, Steve ; Woodward, Wendy A ; Bader, Michael ; Iannaccone, Philip ; Binas, Bert Breant, Bernadette

PloS one, 2009-09, Vol.4 (9), p.e7216-e7216 [Periódico revisado por pares]

United States: Public Library of Science

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Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines
Autor: Debeb, Bisrat G ; Galat, Vasiliy ; Epple-Farmer, Jessica ; Iannaccone, Steve ; Woodward, Wendy A ; Bader, Michael ; Iannaccone, Philip ; Binas, Bert
Breant, Bernadette
Assuntos: Animals ; Biotechnology ; Blastocysts ; Cell Culture Techniques ; Cell Differentiation ; Cell Lineage ; Cell Separation ; Children & youth ; Cloning ; Developmental biology ; Developmental Biology/Cell Differentiation ; Developmental Biology/Molecular Development ; Developmental Biology/Stem Cells ; Embryo cells ; Embryonic Stem Cells - cytology ; Embryos ; Endoderm ; Endoderm - cytology ; Endoderm - metabolism ; Female ; Fetuses ; Fibroblasts ; Flow Cytometry ; Gene expression ; Gene Expression Regulation ; Genes ; Genetic transcription ; Laboratories ; Leukemia ; Leukemia inhibitory factor ; Male ; Membranes ; Mice ; Morphology ; Oct-4 protein ; Octamer Transcription Factor-3 - metabolism ; Placenta ; Precursors ; Rats ; Rodents ; Stem cells ; Transcription factors ; Transcription Factors - metabolism ; Veterinary colleges ; Veterinary medicine ; Yolk ; Yolk sac
É parte de: PloS one, 2009-09, Vol.4 (9), p.e7216-e7216
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Current address: Division of Molecular & Life Sciences, Hanyang University, Ansan-si, Gyeonggi-do, Korea
Current address: Division of Radiation Oncology, University of Texas M.D. Anderson Cancer Center, Houston, Texas, United States of America
Conceived and designed the experiments: BGD BB. Performed the experiments: BGD VG BB. Analyzed the data: BGD VG SI WW MB PI BB. Contributed reagents/materials/analysis tools: BGD VG JEF BB. Wrote the paper: BGD BB. Started the project: BB.
Descrição: The extraembryonic endoderm (ExEn) defines the yolk sac, a set of membranes that provide essential support for mammalian embryos. Recent findings suggest that the committed ExEn precursor is present already in the embryonic Inner Cell Mass (ICM) as a group of cells that intermingles with the closely related epiblast precursor. All ICM cells contain Oct4, a key transcription factor that is first expressed at the morula stage. In vitro, the epiblast precursor is most closely represented by the well-characterized embryonic stem (ES) cell lines that maintain the expression of Oct4, but analogous ExEn precursor cell lines are not known and it is unclear if they would express Oct4. Here we report the isolation and characterization of permanently proliferating Oct4-expressing rat cell lines ("XEN-P cell lines"), which closely resemble the ExEn precursor. We isolated the XEN-P cell lines from blastocysts and characterized them by plating and gene expression assays as well as by injection into embryos. Like ES cells, the XEN-P cells express Oct4 and SSEA1 at high levels and their growth is stimulated by leukemia inhibitory factor, but instead of the epiblast determinant Nanog, they express the ExEn determinants Gata6 and Gata4. Further, they lack markers characteristic of the more differentiated primitive/visceral and parietal ExEn stages, but exclusively differentiate into these stages in vitro and contribute to them in vivo. Our findings (i) suggest strongly that the ExEn precursor is a self-renewable entity, (ii) indicate that active Oct4 gene expression (transcription plus translation) is part of its molecular identity, and (iii) provide an in vitro model of early ExEn differentiation.
Editor: United States: Public Library of Science
Idioma: Inglês

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Isolation of Oct4-expressing extraembryonic endoderm precursor cell lines

Debeb, Bisrat G ; Galat, Vasiliy ; Epple-Farmer, Jessica ; Iannaccone, Steve ; Woodward, Wendy A ; Bader, Michael ; Iannaccone, Philip ; Binas, Bert Breant, Bernadette

United States: Public Library of Science

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