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Isolation and Characterization of the Gene (CLS1 ) Encoding Cardiolipin Synthase in Saccharomyces cerevisiae

Chang, Shao-Chun ; Heacock, Philip N. ; Mileykovskaya, Eugenia ; Voelker, Dennis R. ; Dowhan, William

The Journal of biological chemistry, 1998-06, Vol.273 (24), p.14933-14941 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Isolation and Characterization of the Gene (CLS1 ) Encoding Cardiolipin Synthase in Saccharomyces cerevisiae
Autor: Chang, Shao-Chun ; Heacock, Philip N. ; Mileykovskaya, Eugenia ; Voelker, Dennis R. ; Dowhan, William
Assuntos: ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Amino Acid Sequence ; AMINO ACID SEQUENCES ; BIOCHEMISTRY ; BIOCHIMIE ; BIOQUIMICA ; cardiolipin ; Cardiolipin synthase ; Cardiolipins - biosynthesis ; CATION ; CATIONES ; CATIONS ; CELL MEMBRANES ; CHEMICAL COMPOSITION ; CITOCROMO C OXIDASA ; Cloning, Molecular ; CLS1 GENE ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; CYTOCHEMISTRY ; CYTOCHROME C OXIDASE ; CYTOCHROME C OXYDASE ; DIPHOSPHATIDYLGLYCEROLS ; DNA Mutational Analysis ; ENZIMAS ; ENZYME ; ENZYMES ; ENZYMIC ACTIVITY ; EXPRESION GENICA ; EXPRESSION DES GENES ; FOSFOLIPIDOS ; Fungal Proteins - chemistry ; GAL1 gene ; GENE ; GENE EXPRESSION ; Gene Expression - genetics ; GENES ; HIDROGENO ; HYDROGEN ; HYDROGENE ; ION ; ION TRANSPORT ; IONES ; IONS ; LIPOGENESE ; LIPOGENESIS ; MEMBRANAS CELULARES ; MEMBRANE CELLULAIRE ; Membrane Proteins ; Microscopy, Fluorescence ; MITOCHONDRIA ; MITOCHONDRIE ; MITOCONDRIA ; MOLECULAR SEQUENCE DATA ; NUCLEOTIDE SEQUENCE ; PHOSPHATIDE ; phosphatidylglycerol ; PHOSPHOLIPIDS ; Phospholipids - analysis ; Pyridinium Compounds - metabolism ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - enzymology ; SECUENCIA NUCLEOTIDICA ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEOTIDIQUE ; STRUCTURAL GENES ; Transferases (Other Substituted Phosphate Groups) - chemistry ; Transferases (Other Substituted Phosphate Groups) - genetics
É parte de: The Journal of biological chemistry, 1998-06, Vol.273 (24), p.14933-14941
Notas: 1997094248
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Descrição: In eukaryotic cells, cardiolipin (CL) synthase catalyzes the final step in the synthesis of CL from phosphatidylglycerol and CDP-diacylglycerol. CL and its synthesis are localized predominantly to the mitochondrial inner membrane, and CL is generally thought to be an essential component of many mitochondrial processes. By using homology searches for genes potentially encoding phospholipid biosynthetic enzymes, we have cloned the gene (CLS1 ) encoding CL synthase in Saccharomyces cerevisiae . Overexpression of the CLS1 gene under its endogenous promoter or the inducible GAL1 promoter in yeast and expression of CLS1 in baculovirus-infected insect cells resulted in elevated CL synthase activity. Disruption of theCLS1 gene in a haploid yeast strain resulted in the loss of CL synthase activity, no detectable CL, a 5-fold elevation in phosphatidylglycerol levels, and lack of staining of mitochondria by a dye with high affinity for CL. Thecls1 ::TRP1 null mutant grew on both fermentable and non-fermentable carbon sources but more poorly on the latter. The level and activity of cytochrome c oxidase was normal, and a dye whose accumulation is dependent on membrane proton electrochemical potential effectively stained the mitochondria. These results definitively identify the gene encoding the CL synthase of yeast.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Isolation and Characterization of the Gene (CLS1 ) Encoding Cardiolipin Synthase in Saccharomyces cerevisiae

Chang, Shao-Chun ; Heacock, Philip N. ; Mileykovskaya, Eugenia ; Voelker, Dennis R. ; Dowhan, William

United States: Elsevier Inc

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