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Dopamine- and Tyrosine Hydroxylase-Immunoreactive Neurons in the Brain of the American Cockroach, Periplaneta americana

Hamanaka, Yoshitaka ; Minoura, Run ; Nishino, Hiroshi ; Miura, Toru ; Mizunami, Makoto Tanimoto, Hiromu

PloS one, 2016-08, Vol.11 (8), p.e0160531-e0160531 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Dopamine- and Tyrosine Hydroxylase-Immunoreactive Neurons in the Brain of the American Cockroach, Periplaneta americana
Autor: Hamanaka, Yoshitaka ; Minoura, Run ; Nishino, Hiroshi ; Miura, Toru ; Mizunami, Makoto
Tanimoto, Hiromu
Assuntos: American cockroach ; Anatomy ; Animals ; Antennal lobe ; Antibodies ; Antisera ; Apis mellifera ; Arousal ; Arthropods ; Biology and Life Sciences ; Biotin - analogs & derivatives ; Biotin - pharmacology ; Brain ; Brain - anatomy & histology ; Brain - cytology ; Brain - physiology ; Brain architecture ; Brain research ; Catecholamine ; Catecholamines ; Central nervous system ; Coordination compounds ; Dopamine ; Dopamine - immunology ; Dopamine - metabolism ; Dopamine receptors ; Drosophila ; Environmental science ; Enzymes ; Female ; Gryllus bimaculatus ; Hydroxylase ; Immunoreactivity ; Insect Proteins - immunology ; Insect Proteins - metabolism ; Insects ; Laboratories ; Male ; Medicine and Health Sciences ; Memory ; Neural networks ; Neurons ; Neurons - drug effects ; Neurons - immunology ; Neurons - metabolism ; Neurons - physiology ; Olfactory pathways ; Periplaneta - physiology ; Periplaneta americana ; Physical Sciences ; Physiology ; Proteins ; Studies ; Subdivisions ; Tyrosine ; Tyrosine 3-monooxygenase ; Tyrosine 3-Monooxygenase - immunology ; Tyrosine 3-Monooxygenase - metabolism
É parte de: PloS one, 2016-08, Vol.11 (8), p.e0160531-e0160531
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Current address: Faculty of Science, Osaka University, Toyonaka, Osaka, 560–0043, Japan
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: YH MM.Performed the experiments: YH.Analyzed the data: YH.Contributed reagents/materials/analysis tools: RM HN TM.Wrote the paper: YH HN MM.
Descrição: The catecholamine dopamine plays several vital roles in the central nervous system of many species, but its neural mechanisms remain elusive. Detailed neuroanatomical characterization of dopamine neurons is a prerequisite for elucidating dopamine's actions in the brain. In the present study, we investigated the distribution of dopaminergic neurons in the brain of the American cockroach, Periplaneta americana, using two antisera: 1) an antiserum against dopamine, and 2) an antiserum against tyrosine hydroxylase (TH, an enzyme required for dopamine synthesis), and identified about 250 putatively dopaminergic neurons. The patterns of dopamine- and TH-immunoreactive neurons were strikingly similar, suggesting that both antisera recognize the same sets of "dopaminergic" neurons. The dopamine and TH antibodies intensively or moderately immunolabeled prominent brain neuropils, e.g. the mushroom body (memory center), antennal lobe (first-order olfactory center) and central complex (motor coordination center). All subdivisions of the mushroom body exhibit both dopamine and TH immunoreactivity. Comparison of immunolabeled neurons with those filled by dye injection revealed that a group of immunolabeled neurons with cell bodies near the calyx projects into a distal region of the vertical lobe, which is a plausible site for olfactory memory formation in insects. In the antennal lobe, ordinary glomeruli as well as macroglomeruli exhibit both dopamine and TH immunoreactivity. It is noteworthy that the dopamine antiserum labeled tiny granular structures inside the glomeruli whereas the TH antiserum labeled processes in the marginal regions of the glomeruli, suggesting a different origin. In the central complex, all subdivisions excluding part of the noduli and protocerebral bridge exhibit both dopamine and TH immunoreactivity. These anatomical findings will accelerate our understanding of dopaminergic systems, specifically in neural circuits underlying aversive memory formation and arousal, in insects.
Editor: United States: Public Library of Science
Idioma: Inglês

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Dopamine- and Tyrosine Hydroxylase-Immunoreactive Neurons in the Brain of the American Cockroach, Periplaneta americana

Hamanaka, Yoshitaka ; Minoura, Run ; Nishino, Hiroshi ; Miura, Toru ; Mizunami, Makoto Tanimoto, Hiromu

United States: Public Library of Science

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