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Cre-recombinase systems for induction of neuron-specific knockout models: a guide for biomedical researchers

Shcholok, Tetiana ; Eftekharpour, Eftekhar

Neural regeneration research, 2023-02, Vol.18 (2), p.273-279 [Periódico revisado por pares]

Mumbai: Wolters Kluwer India Pvt. Ltd

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  • Título:
    Cre-recombinase systems for induction of neuron-specific knockout models: a guide for biomedical researchers
  • Autor: Shcholok, Tetiana ; Eftekharpour, Eftekhar
  • Assuntos: central nervous system; cerebellum; cre/loxp system; cre-recombinase transduction; gene deletion; gene delivery; hippocampus; in vivo genome editing; stereotaxic injection ; Review
  • É parte de: Neural regeneration research, 2023-02, Vol.18 (2), p.273-279
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-3
    content type line 23
    ObjectType-Review-1
    Author contributions: TS performed the literature search, drafted the manuscript, drew the illustrations, and provided the tables. EE wrote additional materials, edited, and reviewed the final version. Both authors approved the final version of the manuscript.
  • Descrição: Gene deletion has been a valuable tool for unraveling the mysteries of molecular biology. Early approaches included gene trapping and gene targetting to disrupt or delete a gene randomly or at a specific location, respectively. Using these technologies in mouse embryos led to the generation of mouse knockout models and many scientific discoveries. The efficacy and specificity of these approaches have significantly increased with the advent of new technology such as clustered regularly interspaced short palindromic repeats for targetted gene deletion. However, several limitations including unwanted off-target gene deletion have hindered their widespread use in the field. Cre-recombinase technology has provided additional capacity for cell-specific gene deletion. In this review, we provide a summary of currently available literature on the application of this system for targetted deletion of neuronal genes. This article has been constructed to provide some background information for the new trainees on the mechanism and to provide necessary information for the design, and application of the Cre-recombinase system through reviewing the most frequent promoters that are currently available for genetic manipulation of neurons. We additionally will provide a summary of the latest technological developments that can be used for targeting neurons. This may also serve as a general guide for the selection of appropriate models for biomedical research.
  • Editor: Mumbai: Wolters Kluwer India Pvt. Ltd
  • Idioma: Inglês
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