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Down-regulation of voltage-dependent sodium channels coincides with a low expression of alpha beta sub(1) subunit complexes

Alcaraz, G ; Sampo, B ; Tricaud, N ; Giraud, P ; Martin-Eauclaire, M F ; Couraud, F ; Dargent, B

Brain research. Molecular brain research., 1997-11, Vol.51 (1-2), p.143-153 [Periódico revisado por pares]

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  • Título:
    Down-regulation of voltage-dependent sodium channels coincides with a low expression of alpha beta sub(1) subunit complexes
  • Autor: Alcaraz, G ; Sampo, B ; Tricaud, N ; Giraud, P ; Martin-Eauclaire, M F ; Couraud, F ; Dargent, B
  • É parte de: Brain research. Molecular brain research., 1997-11, Vol.51 (1-2), p.143-153
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    content type line 23
    ObjectType-Feature-1
  • Descrição: The association between the beta 1 subunit and the alpha subunit of the sodium channel from rat brain was studied in hippocampus during postnatal development and in cultures of fetal rat forebrain neurons and cerebellar granule cells, using an anti- beta 1 antipeptide antibody to specifically immunoprecipitate alpha beta 1 complexes labeled with [3H]saxitoxin. In the hippocampus, the increase in beta 1 RNA expression during development was accompanied by an increase in immunoprecipitated alpha beta 1 complexes. Most of the alpha beta 1 complexes were constituted during the first 3 postnatal weeks, with the steepest rise between postnatal days 5 and 12. In cultured fetal neurons, the amount of beta 1 RNA and of alpha beta 1 complexes was approximately 3-4% of that found in the adult, whereas it reached 60-70% in cultured cerebellar granule cells. We had previously described a neurotoxin-induced internalization of sodium channels which occurred in immature neurons but not in adult tissue. Internalization decreased during development in neurotoxin-treated hippocampal slices, and resistance of plasma membrane sodium channels to internalization followed the same time course than the appearance of alpha beta 1 complexes. Similarly, neurotoxin activation resulted in sodium channel internalization in fetal neurons, while cerebellar granule cells, which express high levels of beta 1 RNA and of alpha beta 1 complexes, did not internalize their [3H]saxitoxin receptors in that same conditions. These data suggested that the association of the beta 1 subunit with the alpha subunit could provide a suitable marker for the stabilization and anchoring of sodium channels in discrete membrane domains which occur during neuronal development.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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