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Protein Carbonyl as a Biomarker of Oxidative Stress in Severe Leptospirosis, and Its Usefulness in Differentiating Leptospirosis from Dengue Infections

Fernando, Narmada ; Wickremesinghe, Shalini ; Niloofa, Roshan ; Rodrigo, Chaturaka ; Karunanayake, Lilani ; de Silva, H Janaka ; Wickremesinghe, A R ; Premawansa, Sunil ; Rajapakse, Senaka ; Handunnetti, Shiroma M Khodarahmi, Reza

PloS one, 2016-06, Vol.11 (6), p.e0156085-e0156085 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Protein Carbonyl as a Biomarker of Oxidative Stress in Severe Leptospirosis, and Its Usefulness in Differentiating Leptospirosis from Dengue Infections
Autor: Fernando, Narmada ; Wickremesinghe, Shalini ; Niloofa, Roshan ; Rodrigo, Chaturaka ; Karunanayake, Lilani ; de Silva, H Janaka ; Wickremesinghe, A R ; Premawansa, Sunil ; Rajapakse, Senaka ; Handunnetti, Shiroma M
Khodarahmi, Reza
Assuntos: Acids ; Adult ; Antioxidants ; Antioxidants - metabolism ; Bilirubin ; Biochemistry ; Bioindicators ; Biological markers ; Biology and Life Sciences ; Biomarkers ; Biomarkers - blood ; Biotechnology ; Carbonyls ; Case-Control Studies ; Cytokines ; Damage assessment ; Damage detection ; Dengue ; Dengue - blood ; Dengue - diagnosis ; Dengue - virology ; Dengue fever ; Dengue Virus - isolation & purification ; Development and progression ; Diagnosis ; Diagnosis, Differential ; Disease ; Disease control ; Epidemics ; Fatty acids ; Female ; Health aspects ; Humans ; Immune response ; Infections ; Laboratories ; Leptospira ; Leptospira - isolation & purification ; Leptospirosis ; Leptospirosis - blood ; Leptospirosis - diagnosis ; Leptospirosis - microbiology ; Lipid peroxidation ; Lipids ; Male ; Medicine ; Medicine and Health Sciences ; Molecular biology ; Mortality ; Nitrates ; Nitric oxide ; Oxidation ; Oxidative Stress ; Pathogenesis ; Pathogens ; Patients ; Physical Sciences ; Protein Carbonylation ; Proteins ; Risk factors ; Sepsis ; Serum proteins ; Tropical diseases ; Uric acid ; Vector-borne diseases ; Viral diseases ; Zoonoses
É parte de: PloS one, 2016-06, Vol.11 (6), p.e0156085-e0156085
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Conceived and designed the experiments: SMH SR SP HJdS SW NF. Performed the experiments: NF SW LK. Analyzed the data: NF SMH SR ARW. Contributed reagents/materials/analysis tools: RN. Wrote the paper: NF SMH SR SP HJdS. Critically reviewed and revised the draft, and approved the final manuscript: NF SW RN CR LK HJdS ARW SP SR SMH.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Pathogenesis of disease severity in leptospirosis is not clearly understood whether it is due to direct damage by pathogen or by adverse immune responses. Knowledge on biomarkers of oxidative stress which could be used in identifying patients with severe illness has shown to be of great value in disease management. Thus, the main aim of this study was to assess the damage to serum proteins and lipids, and their significance as biomarkers of oxidative stress in severe leptospirosis. In regions endemic for both leptospirosis and dengue, leptospirosis cases are often misdiagnosed as dengue during dengue epidemics. Therefore, the second aim was to assess the potential of the oxidative stress markers in differentiating severe leptospirosis from critical phase dengue. We measured serum antioxidants (uric acid and bilirubin), total antioxidant capacity (AOC), protein carbonyl (PC) and lipid hydroperoxide (LP) in patients with severe leptospirosis (n = 60), mild leptospirosis (n = 50), dengue during the critical phase (n = 30) and in healthy subjects (n = 30). All patient groups had similar total antioxidant capacity levels. However, the presence of significantly high uric acid and total bilirubin levels may reflect the degree of renal and hepatic involvement seen in severe leptospirosis patients (p<0.02). Serum PC and LP levels were significantly higher in leptospirosis patients compared to critical phase dengue infections (p<0.005). Moreover, high serum PC levels appear to differentiate SL from DC [area under the curve (AUC) = 0.96; p<0.001]. Serum PC may be a reliable biomarker of oxidative damage to serum proteins to identify severe leptospirosis patients (AUC = 0.99) and also to differentiate severe leptospirosis from mild cases (AUC = 0.78; p<0.005) indicating its contribution to pathogenesis. Use of serum PC as an indicator of leptospirosis severity and as an oxidative stress biomarker in differentiating leptospirosis from dengue would provide the opportunity to save lives via prompt patient management.
Editor: United States: Public Library of Science
Idioma: Inglês

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Protein Carbonyl as a Biomarker of Oxidative Stress in Severe Leptospirosis, and Its Usefulness in Differentiating Leptospirosis from Dengue Infections

Fernando, Narmada ; Wickremesinghe, Shalini ; Niloofa, Roshan ; Rodrigo, Chaturaka ; Karunanayake, Lilani ; de Silva, H Janaka ; Wickremesinghe, A R ; Premawansa, Sunil ; Rajapakse, Senaka ; Handunnetti, Shiroma M Khodarahmi, Reza

United States: Public Library of Science

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