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Technical comparison of MinIon and Illumina technologies for genotyping Chikungunya virus in clinical samples

de Souza, Leandro Menezes ; de Oliveira, Isabelle Dias ; Sales, Flávia Cristina Silva ; da Costa, Antonio Charlys ; Campos, Karoline Rodrigues ; Abbud, Adriano ; Guerra, Juliana Mariotti ; dos Santos Cirqueira Borges, Cinthya ; Takahashi, Carlos Pires Fernandes Júnior ; de Araújo, Leonardo José Tadeu

Journal of Genetic Engineering and Biotechnology, 2023-12, Vol.21 (1), p.88-8, Article 88 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Technical comparison of MinIon and Illumina technologies for genotyping Chikungunya virus in clinical samples
Autor: de Souza, Leandro Menezes ; de Oliveira, Isabelle Dias ; Sales, Flávia Cristina Silva ; da Costa, Antonio Charlys ; Campos, Karoline Rodrigues ; Abbud, Adriano ; Guerra, Juliana Mariotti ; dos Santos Cirqueira Borges, Cinthya ; Takahashi, Carlos Pires Fernandes Júnior ; de Araújo, Leonardo José Tadeu
Assuntos: Bioinformatics ; Biomedical Engineering and Bioengineering ; Biotechnology ; Chikungunya virus ; Chikungunya virus genotype ; Construction ; Dengue fever ; DNA sequencing ; Engineering ; Genetic Engineering ; Genetics and Genomics ; Genomes ; Genomics ; Genotypes ; Genotyping ; Metric MinION and Illumina ; Next-generation sequencing ; Nucleotide sequencing ; Proteomics ; Public health ; Software ; Surveillance ; Viruses
É parte de: Journal of Genetic Engineering and Biotechnology, 2023-12, Vol.21 (1), p.88-8, Article 88
Descrição: New-generation sequencing (NGS) techniques have brought the opportunity for genomic monitoring of several microorganisms potentially relevant to public health. The establishment of different methods with different mechanisms provides a wide choice, taking into account several aspects. With that in mind, the present aim of the study was to compare basic genomic sequencing metrics that could potentially impact genotyping by nanopores from Oxford Nanopore Technologies and by synthesis from Illumina in clinical samples positive for Chikungunya (CHIKV). Among the metrics studied, running time, read production, and Q score were better represented in Illumina sequencing, while the MinIOn platform showed better response time and greater diversity of generated files. That said, it was possible to establish differences between the studied metrics in addition to verifying that the distinctions in the methods did not impact the identification of the CHIKV virus genotype.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Technical comparison of MinIon and Illumina technologies for genotyping Chikungunya virus in clinical samples

Berlin/Heidelberg: Springer Berlin Heidelberg

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